ALAD Antibody

Western Blot: ALAD Antibody [H00000210-D01P] - Analysis of ALAD expression in human liver.

Western Blot: ALAD Antibody [H00000210-D01P] - Analysis of ALAD expression in transfected 293T cell line by ALAD polyclonal antibody.Lane 1: ALAD transfected lysate(37.20 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

ALAD Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ALAD (NP_000022.2, 1 a.a. - 339 a.a.) full-length human protein. MPLCPLAHAMQPQSVLHSGYFHPLLRAWQTATTTLNASNLIYPIFVTDVPDDIQPITSLPGVARYGVKRLEEMLRPLVEEGLRCVLIFGVPSRVPKDERGSAADSEESPAIEAIHLLRKTFPNLLVACDVCLCPYTSHGHCGLLSENGAFRAEESRQRLAEVALAYAKAGCQVVAPSDMMDGRVEAIKEALMAHGLGNRVSVMSYSAKFASCFYGPFRDAAKSSPAFGDRRCYQLPPGARGLALRAVDRDVREGADMLMVKPGMPYLDIVREVKDKHPDLPLAVYHVSGEFAMLWHGAQAGAFDLKAAVLEAMTAFRRAGADIIITYYTPQLLQWLKEE
• Specificity: Reacts with aminolevulinate, delta-, dehydratase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ALAD
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody is reactive against tissue and transfected lysate in WB and as a detection antibody in ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ALAD Antibody

• ALADHEC 4.2.1.24
• aminolevulinate dehydratase
• aminolevulinate, delta-, dehydratase
• delta-aminolevulinic acid dehydratase
• PBGSMGC5057
• Porphobilinogen synthase

Background

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALAD Antibody (H00000210-D01P)(1)

Publication using H00000210-D01P

• Anand S, Wilson C, Hasan T, Maytin EV. Vitamin D3 enhances the apoptotic response of epithelial tumors to aminolevulinate-based photodynamic therapy. Cancer Res. 2011-08-01 [PMID: 21807844]

Bioinformatics

• Gene Symbol: ALAD
• Entrez:
  • 210(Human)
• Uniprot:
  • NP_000022.2(Human)