AKT1/2/3 Inhibitor Peptide Set

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Product Details

Summary
Reactivity HuSpecies Glossary
Concentration
LYOPH

Order Details

AKT1/2/3 Inhibitor Peptide Set Summary

Immunogen
Functions as a TCL1 PH decoy by binding to Akt.
Specificity
The Akt (Isoforms 1,2,3) inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.

The control peptide consists of only the PTD sequence.
Preparation
Method
Preparation of 5 mM Stock Solutions
PBS* is added directly to the vials to prepare the stock solutions.
Note: Bring the solution to room temperature and quick spin the tubes before opening the caps. Akt (Isoforms 1,2,3) Inhibitor Peptide: 1 mg of DRQIKIWFQNRRMKWKKAVTDHPDRLWAWEKF Add 47.6 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.

Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
Recipe for 1X PBS:
1. Dissolve the following in 800ml distilled H2O.
-8g of NaCl
-0.2g of KCl
-1.44g of Na2HPO4
-0.24g of KH2PO4
2. Adjust pH to 7.5 with HCl.
3. Adjust volume to 1L with additional distilled H2O.
4. Sterilize by autoclaving
Content
Akt (Isoforms 1,2,3) Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKAVTDHPDRLWAWEKF (AKT sequence: AVTDHPDRLWAWEKF). Molecular weight: 4214. Antennapedia Control peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKK. Molecular weight: 2361
Gene
AKT1

Applications/Dilutions

Application Notes
Inhibition of Akt kinase activity.
The inhibitor peptide is to block Akt1, Akt2, and Akt3 kinase activity. Optimal peptide concentrations and incubation times vary between model systems and should be determined empirically by users. A 100 uM final concentration may be a useful starting point.

Please refer to Hiromura et al (2004) for additional information about how the inhibitor peptide has been used to block Ak1, Akt2 and Akt3 kinase activity.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-29332 in the following application:

Publications
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NBP2-29332 in the following applications:

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Solubilize the peptides prior to use by making 5 mM PBS* stock solutions (please see Preparation of 5 mM Stock Solutions under Preparation Method).
Concentration
LYOPH
Reconstitution Instructions
Please contact technical support for detailed reconstitution instructions.

Alternate Names for AKT1/2/3 Inhibitor Peptide Set

  • AKT
  • EC 2.7.11
  • EC 2.7.11.1
  • PKBMGC99656
  • PRKBA
  • Protein kinase B
  • Proto-oncogene c-Akt
  • rac protein kinase alpha
  • RAC-alpha serine/threonine-protein kinase
  • RAC-ALPHA
  • RAC-PK-alpha
  • RACPKB-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1

Background

Akt is a protein kinase that plays a central role in inhibiting apoptosis through promoting cell survival. Activated Akt functions by phosphorylating downstream targets in survival signaling pathways. TCL1 (a proto-oncogene underlying human T cell prolymphocytic leukemia) interacts with Akt through an Nterminal pleckstrin homology (PH) domain and functions as an Akt kinase co-activator. This inhibitory peptide contains a sequence (AVTDHPDRLWAWEKF) corresponding to the A strand of human TCL1 that interacts with Akt. 1 The peptide binds to the PH domain of Akt and inhibits Akt1, Akt2, and Akt3 kinase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

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Publications for AKT1/2/3 Inhibitor (NBP2-29332)(2)

Review for AKT1/2/3 Inhibitor (NBP2-29332) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-29332:
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Images Ratings Applications Species Date Details
Western Blot AKT1/2/3 NBP2-29332
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reviewed by:
Ronald Miller
WB Human 12/12/2014
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Summary

ApplicationWestern Blot
Sample TestedCell lysate from HeLa cells
SpeciesHuman

Blocking

Blocking Details5% BSA in 1X PBS

Primary Anitbody

Dilution Ratio1:1000 in 1X PBS with troton X-100 (0.1%) at 4 degree O/N

Secondary Antibody

Secondary DescriptionGoat Anti-mouse IgG - HRP (Life Tech)
Secondary Manufacturer Cat#A24524
Secondary Concentration1:5K

Details

Detection NotesECL, 1 min exposure with film

Comments

CommentsThe inhibitor works well but I would go for siRNA as it gives a better silencing.

Product General Protocols

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FAQs for AKT1/2/3 Inhibitor (NBP2-29332) (0)

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Other Available Formats

NBP2-29332
NBP2-29332

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1
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Ronald Miller
12/12/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol AKT1
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