AKT1/2/3 Antibodies
We offer AKT1/2/3 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each AKT1/2/3 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our AKT1/2/3 Antibodies can be used in a variety of model species: Human, Mouse, Rat. Use the list below to choose the AKT1/2/3 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our AKT1/2/3 polyclonal antibodies.
Alternate Names for AKT1/2/3 Antibodies
anti-AKT1/2/3 antibody, anti-AKT1 antibody, anti-AKT antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.1 antibody, anti-PKBMGC99656 antibody, anti-PRKBA antibody, anti-Protein kinase B antibody, anti-Proto-oncogene c-Akt antibody, anti-rac protein kinase alpha antibody, anti-RAC-ALPHA antibody, anti-RAC-alpha serine/threonine-protein kinase antibody, anti-RAC-PK-alpha antibody, anti-RACPKB-ALPHA antibody, anti-v-akt murine thymoma viral oncogene homolog 1 antibody
5 Results for "akt1-2-3" in Primary Antibodies
![Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231526123.jpg "Immunohistochemistry: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunohistochemical analysis of paraffin-embedded human testis, using NBP3-21678.")
![Immunocytochemistry/Immunofluorescence: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Immunofluorescent analysis of MCF-7 cells, using NBP3-21678.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-57202317161873.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2006) [NBP3-21678] - Western blot analysis using NBP3-21678 in (1) A549 cell lysate; (2) Mouse brain lysate; (3) Rat brain lysate; (4) Rat heart lysate; (5) Rat kidney lysate.](http://images.novusbio.com/fullsize/nbp3-21678_rabbit-akt1-2-3-mab-sr2006-5720231558486.jpg)
![Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22614_rabbit-akt1-2-3-mab-sr2003-57202317215156.jpg "Western Blot: AKT1/2/3 Antibody (SR2003) [NBP3-22614] - Western blot analysis using NBP3-22614 in (1) Hela cell lysate;(2) A549 cell lysate.")
![Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202410334024.jpg "Immunocytochemistry/ Immunofluorescence: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Immunocytochemistry analysis of MCF7 cells labeling AKT1/2/3 with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Flow cytometric analysis of MCF7 cells labeling AKT1/2/3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32017, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-29202416453243.jpg)
![Western Blot: AKT1/2/3 Antibody (JE75-09) [NBP3-32017] - Western blot analysis of AKT1/2/3 on different lysates with Rabbit anti-AKT1/2/3 antibody (NBP3-32017) at 1/2,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: A549 cell lysateLane 4: U-2 OS cell lysateLane 5: NIH/3T3 cell lysateLysates/proteins at 15 µg/Lane.Predicted band size: 56 kDaObserved band size: 56 kDaExposure time: 30 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32017_rabbit-akt1-2-3-mab-je75-09-3920248453538.jpg)
![Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-2722024847194.jpg "Immunohistochemistry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Image of AKT1/2/3 Antibody (16E10) diluted at 1:50 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.54% DAB.")
![Western Blot: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Positive Western Blot detected in: SH-SY5Y whole cell lysate. All lanes: AKT1/2/3 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 56 kDa Observed band size: 56 kDa](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-282202411442636.jpg)
![Flow Cytometry: AKT1/2/3 Antibody (16E10) [NBP3-26514] - Overlay Peak curve showing Jurkat cells stained with AKT1/2/3 Antibody (16E10) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26514_rabbit-akt1-2-3-mab-16e10-28220241418367.jpg)
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0001.jpg "Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of various cell lines, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: AKT1/2/3 [p Ser473] Antibody (2E3A3) [NBP3-16948] - Analysis of extracts of NIH/3T3 cells, using Phospho-Akt-S473 antibody (NBP3-16948) at 1:1000 dilution.NIH/3T3 cells were treated by Calyculin A (100 nM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AKT1-2-3-p-Ser473-Antibody-2E3A3-Western-Blot-NBP3-16948-img0002.jpg)