Recombinant Human AKR1D1 Protein

Product Discontinued

Recombinant Human AKR1D1 Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-326 of Human AKR1D1
  
  

  Source: E.coli

  Amino Acid Sequence: MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNPIW VNVSSPPLLK DALLNSLGKR YNKTAAQIVL RFNIQRGVVV IPKSFNLERI KENFQIFDFS LTEEEMKDIE ALNKNVRFVE LLMWRDHPEY PFHDEY

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AKR1D1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 37.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.5), 1 mM DTT, 0.1 M NaCl, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human AKR1D1 Protein

• 3o5bred
• Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase
• aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase)
• CBAS2
• Delta(4)-3-oxosteroid 5-beta-reductase
• EC 1.3.1.3
• SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)

Background

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for AKR1D1 Recombinant Protein (NBP2-52292)(1)

Publication using NBP2-52292

• Valanejad L, Ghareeb M, Shiffka S et al. Dysregulation of d(4)-3-oxosteroid 5b-reductase in diabetic patients: Implications and mechanisms. Mol. Cell. Endocrinol. 2017-10-09 [PMID: 29024782] (Human)

Bioinformatics

• Gene Symbol: AKR1D1