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AKR1D1 Overexpression Lysate

Images

 
Western Blot: AKR1D1 Overexpression Lysate (Adult Normal) [NBL1-07438] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for AKR1D1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

AKR1D1 Overexpression Lysate Summary

Description
Transient overexpression lysate of aldo-keto reductase family 1, member D1 (delta 4-3-ketosteroid-5-beta-reductase) (AKR1D1) The lysate was created in HEK293T cells, using Plasmid ID RC223056 and based on accession number NM_005989. The protein contains a C-MYC/DDK Tag.
Gene
AKR1D1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
37.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for AKR1D1 Overexpression Lysate

  • 3o5bred
  • Aldo-keto reductase family 1 member D1,3-oxo-5-beta-steroid 4-dehydrogenase
  • aldo-keto reductase family 1, member D1 (delta4-3-ketosteroid-5-beta-reductase)
  • CBAS2
  • Delta(4)-3-oxosteroid 5-beta-reductase
  • EC 1.3.1.3
  • SRD5B1beta polypeptide 1 (3-oxo-5 beta-steroid delta4-dehydrogenase beta 1)

Background

The enzyme encoded by this gene is responsible for the catalysis of the 5-beta-reduction of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure. Deficiency of this enzyme may contribute to hepatic dysfunction. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol AKR1D1