AKAP3 Antibody

Western Blot: AKAP3 Antibody [NB100-1411] - (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AKAP3 Antibody Summary

• Immunogen: Peptide with sequence C-TPLQLLDWLMVNL corresponding to C-Terminus according to NP_006413.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 90 kDa band observed in human testis lysates (calculated MW of 95 kDa band according to NP_006413).
• Theoretical MW: 95 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AKAP3 Antibody

• A kinase (PRKA) anchor protein 3
• AKAP110AKAP 110
• AKAP-3
• A-kinase anchor protein 110 kDa
• Cancer/testis antigen 82
• CT82A-kinase anchor protein 3
• Fibrous sheath protein of 95 kDa
• fibrousheathin 1
• Fibrousheathin I
• fibrousheathin-1
• FSP95A-kinase anchor protein, 110kDa
• PRKA3
• protein kinase A anchoring protein 3
• Protein kinase A-anchoring protein 3
• SOB1fibrous sheath protein, 95kDa
• sperm oocyte-binding protein 1
• Sperm oocyte-binding protein

Background

A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP3 Antibody (NB100-1411)(1)

Publication using NB100-1411

• Lefevre A, Duquenne C, Rousseau-Merck MF et al. Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition. Biochem Biophys Res Commun 1999-05-27 [PMID: 10334916]

Bioinformatics

• Gene Symbol: AKAP3
• Entrez:
  • 10566(Human)
• Uniprot:
  • O75969(Human)