AKAP3 Antibody

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Western Blot: AKAP3 Antibody [NB100-1411] - (1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AKAP3 Antibody Summary

Immunogen
Peptide with sequence C-TPLQLLDWLMVNL corresponding to C-Terminus according to NP_006413.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AKAP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 90 kDa band observed in human testis lysates (calculated MW of 95 kDa band according to NP_006413).
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Testis Lysate (NB820-59266)
Publications
Read Publication using NB100-1411.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKAP3 Antibody

  • A kinase (PRKA) anchor protein 3
  • AKAP110AKAP 110
  • AKAP-3
  • A-kinase anchor protein 110 kDa
  • Cancer/testis antigen 82
  • CT82A-kinase anchor protein 3
  • Fibrous sheath protein of 95 kDa
  • fibrousheathin 1
  • Fibrousheathin I
  • fibrousheathin-1
  • FSP95A-kinase anchor protein, 110kDa
  • PRKA3
  • protein kinase A anchoring protein 3
  • Protein kinase A-anchoring protein 3
  • SOB1fibrous sheath protein, 95kDa
  • sperm oocyte-binding protein 1
  • Sperm oocyte-binding protein

Background

A-kinase anchor proteins (AKAPs) direct the activity of protein kinase A (PKA) by tethering the enzyme near its physiologic substrates. May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction in sperm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP3 Antibody (NB100-1411)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional AKAP3 Products

AKAP3 NB100-1411

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Bioinformatics

Gene Symbol AKAP3
Entrez
Uniprot