AKAP12 Antibody - BSA Free

Western Blot: AKAP12 Antibody [NBP2-66604] - Western blotting of total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyzed. The protein was transferred to a PVDF membrane, blocked with 1X TBS, 0.1% Tween 20/ 5% NFDM, probed with 5 ug/mL of pre-immune serum 5 ug of AKAP12 antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP at 1:10000, and detected with luminol.

• Reactivity: Rt
• Applications: WB, ELISA, AP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Format: BSA Free

AKAP12 Antibody - BSA Free Summary

• Description: Novus Biologicals Sheep AKAP12 Antibody - BSA Free (NBP2-66604) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Sheep were immunized with a recombinant protein corresponding to amino acids 620-1596 of the SSeCKS protein (Uniprot: Q62766).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: AKAP12
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Western Blot 1:100-1:2000
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.08% Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for AKAP12 Antibody - BSA Free

• A kinase (PRKA) anchor protein (gravin) 12
• A kinase (PRKA) anchor protein 12
• AKAP 250
• AKAP250250kDa
• DKFZp686M0430
• DKFZp686O0331
• FLJ20945
• FLJ97621
• Gravin
• kinase scaffold protein gravin
• myasthenia gravis autoantigen gravin
• Myasthenia gravis autoantigen

Background

Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation and proliferation, yet little is known about downstream substrates or their roles in normal or diseased states. AKAP12 or SSeCKS, a novel protein kinase C substrate originally isolated as a src-suppressed negative mitogenic regulator in fibroblasts, controls actinbased cytoskeletal architecture and scaffolds key signaling kinases such as protein kinase C and protein kinase A. Activation of protein kinase C is a key signal transduction event in mesangial cell dedifferentiation. A role for AKAP12, a PKA/PKC scaffolding protein, has been implicated during the process of spermiogenesis and in the actin-based stellate morphology of mesangial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKAP12