AKAP12 Antibody

Images

 
Western Blot: AKAP12 Antibody [NB100-788] - NB100-788 (0.03ug/ml) staining of HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AKAP12 Antibody Summary

Immunogen
Peptide with sequence C-KQERESAKSELTES corresponding to C-Terminus according to NP_005091.2, NP_653080.1.
Specificity
This anrtibody is expected to recognize both reported isoforms.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AKAP12
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.02 - 0.1 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 200+150 kDa band bands observed in human uterus lysates (calculated MW of 191 kDa band according to NP_005091.2 and 181 kDa band according to NP_653080.1).
Control
HeLa Whole Cell Lysate
AKAP12 Overexpression Lysate
Publications
Read Publication using NB100-788.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AKAP12 Antibody

  • A kinase (PRKA) anchor protein (gravin) 12
  • A kinase (PRKA) anchor protein 12
  • AKAP 250
  • AKAP250250kDa
  • DKFZp686M0430
  • DKFZp686O0331
  • FLJ20945
  • FLJ97621
  • Gravin
  • kinase scaffold protein gravin
  • myasthenia gravis autoantigen gravin
  • Myasthenia gravis autoantigen

Background

A kinase anchoring protein thought to be involved the localization of protein kinase A and protein kinase C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AKAP12 Antibody (NB100-788)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics

Gene Symbol AKAP12
Entrez
Uniprot