AKAP12 Antibody

Western Blot: AKAP12 Antibody [NB100-788] - NB100-788 (0.03ug/ml) staining of HeLa cell lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Discontinued

AKAP12 Antibody Summary

• Immunogen: Peptide with sequence C-KQERESAKSELTES corresponding to C-Terminus according to NP_005091.2, NP_653080.1.
• Specificity: This anrtibody is expected to recognize both reported isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AKAP12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.02 - 0.1 ug/ml
• Application Notes: WB: Approx. 200+150 kDa band bands observed in human uterus lysates (calculated MW of 191 kDa band according to NP_005091.2 and 181 kDa band according to NP_653080.1).
• Control:

  HeLa Whole Cell Lysate
  AKAP12 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AKAP12 Antibody

• A kinase (PRKA) anchor protein (gravin) 12
• A kinase (PRKA) anchor protein 12
• AKAP 250
• AKAP250250kDa
• DKFZp686M0430
• DKFZp686O0331
• FLJ20945
• FLJ97621
• Gravin
• kinase scaffold protein gravin
• myasthenia gravis autoantigen gravin
• Myasthenia gravis autoantigen

Background

A kinase anchoring protein thought to be involved the localization of protein kinase A and protein kinase C.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP12 Antibody (NB100-788)(1)

Publication using NB100-788

• Nauert JB, Klauck TM, Langeberg LK, Scott JD. Gravin, an autoantigen recognized by serum from myasthenia gravis patients, is a kinase scaffold protein. Curr Biol 1997-01-01 [PMID: 9000000]

Bioinformatics

• Gene Symbol: AKAP12
• Entrez:
  • 9590(Human)
• Uniprot:
  • NP_005091(Human)