AKAP12 Antibody (1C5) - Azide and BSA Free

Sandwich ELISA: AKAP12 Antibody (1C5) [H00009590-M01] - Detection limit for recombinant GST tagged AKAP12 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

AKAP12 Antibody (1C5) - Azide and BSA Free Summary

• Immunogen: AKAP12 (NP_005091, 1675 a.a. ~ 1782 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VPEDDGHALLAERIEKSLVEPKEDEKGDDVDDPENQNSALADTDASGGLTKESPDTNGPKQKEKEDAQEVELQEGKVHSESDKAITPQAQEELQKQERESAKSELTES
• Specificity: AKAP12 - A kinase (PRKA) anchor protein (gravin) 12
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AKAP12
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AKAP12 Antibody (1C5) - Azide and BSA Free

• A kinase (PRKA) anchor protein (gravin) 12
• A kinase (PRKA) anchor protein 12
• AKAP 250
• AKAP250250kDa
• DKFZp686M0430
• DKFZp686O0331
• FLJ20945
• FLJ97621
• Gravin
• kinase scaffold protein gravin
• myasthenia gravis autoantigen gravin
• Myasthenia gravis autoantigen

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AKAP12 Antibody (H00009590-M01)(2)

Publications using H00009590-M01

• Lin Qifeng, Tan Hwee Tong, Lim Teck Kwang et al. iTRAQ analysis of colorectal cancer cell lines suggests Drebrin (DBN1) is overexpressed during liver metastasis. Proteomics. 2014-04-17 [PMID: 24610677] (WB, Human)
• Tan HT, Tan S, Lin Q et al. Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells. Mol Cell Proteomics 2008-03-14 [PMID: 18344232]

Bioinformatics

• Gene Symbol: AKAP12
• Entrez:
  • 9590(Human)
• OMIM:
  • 604698(Human)
• Uniprot:
  • NP_005091(Human)