AKAP10 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: AKAP10 Antibody [NBP1-31483] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: AKAP10 Antibody [NBP1-31483] - Mouse brain. AKAP10 antibody [C3], C-term diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: AKAP10 Antibody [NBP1-31483] - Human gastric cancer, using AKAP10 antibody at 1:100 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: AKAP10 Antibody [NBP1-31483] - Human gastric cancer, using AKAP10 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: AKAP10 Antibody [NBP1-31483] - Rat liver. AKAP10 antibody [C3], C-term diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AKAP10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AKAP10 Antibody - BSA Free (NBP1-31483) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human AKAP10. The exact sequence is proprietary.
• Localization: Mitochondrion, Membrane, Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AKAP10
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for AKAP10 Antibody - BSA Free

• A kinase (PRKA) anchor protein 10
• AKAP-10
• A-kinase anchor protein 10, mitochondrial
• D-AKAP2
• D-AKAP-2
• Dual specificity A kinase-anchoring protein 2
• dual-specificity A-kinase anchoring protein 2
• MGC9414
• mitochondrial A kinase PPKA anchor protein 10
• PRKA10a kinase anchor protein 10, mitochondrial
• protein kinase A anchoring protein 10
• Protein kinase A-anchoring protein 10

Background

The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: AKAP10