Ajuba Antibody - BSA Free

Western Blot: Ajuba Antibody [NB600-468] - Analysis of a 57-kDa band consistent with the expected MW for Ajuba (arrowhead). Lanes correspond to 1) HeLa nuclear extract, and 2) HeLa, 3) A431, 4) Jurkat and 5) 293 whole cell lysates. Immunoprecipitation of Ajuba followed by western blotting may result in cleaner background staining. Approximately 5 ug of each preparation was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-Ajuba antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 60-sec exposure time.

Western Blot: Ajuba Antibody [NB600-468] - Analysis of Ajuba-RFP fusion protein in cell lysates (arrow-head). Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 ug of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody. Detection occurred using a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Ajuba Antibody - BSA Free Summary

• Description: This affinity purified antibody is directed against human Ajuba. The product was affinity purified from antiserum by immunoaffinity purification
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified Ajuba Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 224-239 of Human Ajuba. (Uniprot: Q96IF1)
• Localization: Cytoplasm, cytoskeleton. Cell membrane. Cell junction. Nucleus. Centrosome. Note: Shuttles between the cytoplasm and the nucleus.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AJUBA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:20000-1:80000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2500
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57 kDa in size corresponding to AJUBA by western blotting in the appropriate cell lysate or extract.
• Control:

  Ajuba Overexpression Lysate

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, mouse and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Ajuba protein homologues from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Ajuba Antibody - BSA Free

• Ajuba
• JUB
• jub, ajuba homolog (Xenopus laevis)
• MGC15563
• protein ajuba

Background

Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AJUBA
• Entrez:
  • 84962(Human)
  • 85265(Rat)
• Uniprot:
  • Q96IF1()
  • Q96IF1()
  • Q96IF1()
  • Q96IF1()