FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." class="big_lightbox">
FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog #
FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG
2BFlow Cytometry Isotype Control (Catalog #
MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog #
FC012)." class="big_thumb" />
FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." alt="Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human Aiolos/IKZF3 Monoclonal Antibody (Catalog # MAB8625) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)." class="big_thumb" />
![Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Western-Blot-NBP2-24495-img0005.jpg "Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Total protein from Jurkat cells and human tonsil was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-IkZF3 in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP2-24495] - IKZF3 antibody was tested in Jurkat cells at a 1:40 dilution against DyLight 488 (Green). Alpha tubulin and nuclei were counterstained against DyLight 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24495-img0003.jpg)
![Western Blot: Aiolos/IKZF3 Antibody [NBP2-24495] - Analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.25 ug/mL.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Western-Blot-NBP2-24495-img0004.jpg)
![Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of various cell lines, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-4V2Z2-Western-Blot-NBP3-16668-img0009.jpg "Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of various cell lines, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of Raji cells, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-4V2Z2-Western-Blot-NBP3-16668-img0008.jpg)
![Western Blot: Aiolos/IKZF3 Antibody (4V2Z2) [NBP3-16668] - Western blot analysis of extracts of Rat lung, using Aiolos/IKZF3 antibody (NBP3-16668) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-4V2Z2-Western-Blot-NBP3-16668-img0010.jpg)
![Immunoprecipitation: Aiolos/IKZF3 Antibody (4U7F9) [NBP3-16668] - Immunoprecipitation analysis of 300 ug extracts of Raji cells using 3 ug Aiolos/IKZF3 antibody . Western blot was performed from the immunoprecipitate using Aiolos/IKZF3 antibody at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-16668_rabbit-aiolos-ikzf3-mab-4u7f9-5220251215441.jpg)
![Immunohistochemistry: Aiolos/IKZF3 Antibody (4U7F9) [NBP3-16668] - Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Aiolos/IKZF3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16668_rabbit-aiolos-ikzf3-mab-4u7f9-522025125426.jpg)
![Immunohistochemistry: Aiolos/IKZF3 Antibody (4U7F9) [NBP3-16668] - Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using Aiolos/IKZF3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16668_rabbit-aiolos-ikzf3-mab-4u7f9-522025125453.jpg)
![Immunohistochemistry: Aiolos/IKZF3 Antibody (4U7F9) [NBP3-16668] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Aiolos/IKZF3 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16668_rabbit-aiolos-ikzf3-mab-4u7f9-52202511374166.jpg)
![Western Blot: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKZF3.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-7E11-Western-Blot-NBP2-46048-img0004.jpg "Western Blot: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IKZF3.")
![Immunohistochemistry: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-7E11-Immunohistochemistry-NBP2-46048-img0003.jpg)
![Immunohistochemistry: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-7E11-Immunohistochemistry-NBP2-46048-img0001.jpg)
![Immunohistochemistry: Aiolos/IKZF3 Antibody (7E11) [NBP2-46048] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-7E11-Immunohistochemistry-NBP2-46048-img0002.jpg)
![Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKZF3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16938_rabbit-polyclonal-aiolos-ikzf3-antibody-1610202419405767.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Aiolos/IKZF3 Antibody [NBP2-16938] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKZF3 antibody [N1C2] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Western Blot: Aiolos/IKZF3 Antibody [NBP3-16978] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Western-Blot-NBP3-16978-img0005.jpg "Western Blot: Aiolos/IKZF3 Antibody [NBP3-16978] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG")
![Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP3-16978] - Analysis in human tonsil and cerebral cortex tissues using Anti-IKZF3 antibody. Corresponding IKZF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16978-img0002.jpg)
![Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody [NBP3-16978] - Staining of human tonsil shows high expression.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunohistochemistry-Paraffin-NBP3-16978-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP3-16978] - Staining of human cell line RT4 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-16978-img0001.jpg)
![Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody [NBP3-16978] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunohistochemistry-Paraffin-NBP3-16978-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP1-89185] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89185-img0008.jpg "Immunocytochemistry/Immunofluorescence: Aiolos/IKZF3 Antibody [NBP1-89185] - Staining of human cell line U-251 MG shows localization to plasma membrane and cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody [NBP1-89185] - Staining in human tonsil and cerebral cortex tissues using anti-IKZF3 antibody. Corresponding IKZF3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunohistochemistry-Paraffin-NBP1-89185-img0007.jpg)
![Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody [NBP1-89185] - Staining of human tonsil shows distinct nuclear positivity in most cells.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunohistochemistry-NBP1-89185-img0003.jpg)
![Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody [NBP1-89185] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-Immunohistochemistry-Paraffin-NBP1-89185-img0006.jpg)
![Western Blot: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Western blot detection of Aiolos/IKZF3 in HL-60, U251 cell lysates using NBP3-20069 (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-S01-0C5-Western-Blot-NBP3-20069-img0002.jpg "Western Blot: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Western blot detection of Aiolos/IKZF3 in HL-60, U251 cell lysates using NBP3-20069 (1:1000 diluted). Predicted band size: 58kDa. Observed band size: 58kDa.")
![Immunohistochemistry-Paraffin: Aiolos/IKZF3 Antibody (S01-0C5) [NBP3-20069] - Immunohistochemistry of Aiolos/IKZF3 in paraffin-embedded Human tonsil using NBP3-20069 at dilution 1/50](http://images.novusbio.com/fullsize/Aiolos-IKZF3-Antibody-S01-0C5-Immunohistochemistry-Paraffin-NBP3-20069-img0001.jpg)
