AICL/CLEC-2B Antibody (1064935) [Unconjugated]

U937 cells were stained with Mouse Anti-Human AICL/CLEC-2B Monoclonal Antibody (Catalog # MAB9059, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 1064935
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

AICL/CLEC-2B Antibody (1064935) [Unconjugated] Summary

• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human AICL/CLEC-2B
  Lys26-His149
  Accession # Q92478
• Specificity: Detects human AICL/CLEC-2B in direct ELISA.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AICL/CLEC-2B Antibody (1064935) [Unconjugated]

• Activation-induced C-type lectin
• AICL
• AICLC-type lectin superfamily member 2
• CLEC2B
• CLEC-2B
• CLECSF2
• CLECSF2IFN-alpha-2b-inducing-related protein 1
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 2 (activation-induced)
• C-type lectin domain family 2 member B
• C-type lectin domain family 2, member B
• HP10085
• IFN-alpha2b-inducing related protein 1
• IFNRG1

Background

Activation-induced C-type lectin (AICL), also known as CLEC2B, is a 30-35 kDa variably glycosylated type-2 transmembrane member of the C-type lectin-like receptor (CTLR) family. AICL belongs to the subgroup of CLEC2 proteins that also includes CLEC2A/KACL, CLEC2D/LLT, and CD69/CLEC2C, all of which are encoded by the natural killer gene complex (1). Human AICL contains a single C-type lectin domain in its extracellular region and a 7 amino acid cytoplasmic tail (2). AICL is expressed on monocytes, macrophages, and granulocytes (3), and it is upregulated on TLR-activated monocytes and IL-12 + IL-18 activated NK cells (3, 4). AICL is an activating receptor that triggers TNF production by monocytes (3). It binds to NKp80 on NK cells, resulting in NK cell mediated lysis of the AICL expressing monocyte (3). In addition, the AICL-NKp80 axis mediates interactions between activated and resting NK cells (4).

1. Li, Y. et al. (2014) Front. Immunol. 5:123.
2. Hamann, J. et al. (1997) Immunogenetics 45:295.
3. Welte, S. et al. (2006) Nat. Immunol. 7:1334.
4. Klimosch, S.N. et al. (2013) Blood 122:2380.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q92478()