AICDA Antibody (359218)

AICDA was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Rat Anti-Human AICDA Monoclonal Antibody (Catalog # MAB39102) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 359218
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

AICDA Antibody (359218) Summary

• Immunogen: E. coli-derived recombinant human AICDA
  Met1-Leu198
  Accession # Q9GZX7
• Specificity: Detects human AICDA in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: AICDA
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AICDA Antibody (359218)

• activation-induced cytidine deaminase
• AICDA
• AID
• AIDCytidine aminohydrolase
• Arp2
• CDA2
• HIGM2
• HIGM2EC 3.5.4.5
• integrated into Burkitt's lymphoma cell line Ramos

Background

AICDA, also known as AID, is a 24 kDa cytidine deaminase that converts deoxycytidine to deoxyuridine in single stranded DNA. Its expression is restricted to activated lymph node and tonsillar B cells and is required for terminal B cell differentiation. Loss of function mutations of AICDA cause severe humoral immunodeficiency by preventing immunoglobulin class switching and somatic hypermutation. Human AICDA shares 92% - 95% amino acid sequence identity with bovine, canine, and mouse AICDA.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AICDA Antibody (MAB39102)(1)

Publication using MAB39102

• H Li, Q Li, Z Ma, Z Zhou, J Fan, Y Jin, Y Wu, F Cheng, P Liang AID modulates carcinogenesis network via DNA demethylation in bladder urothelial cell carcinoma Cell Death Dis, 2019;10(4):251. 2019 [PMID: 30874539] (IHC-P, Human)

Bioinformatics

• Gene Symbol: AICDA
• Uniprot:
  • Q9GZX7()