AHSA1 Antibody (4H9-D7)

Western Blot: AHSA1 Antibody (4H9-D7) [NBP2-12891] - analysis of Human HeLa cell lysates showing detection of Aha1 protein using Mouse Anti-Aha1 Monoclonal Antibody, Clone 4H9.D7 . Primary Antibody: Mouse Anti-Aha1 Monoclonal Antibody at 1:1000. (1) HeLa cell lysate (2) recombinant Aha1 cell lysate.

Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (4H9-D7) [NBP2-12891] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-AHA1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clone: 4H9-D7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

AHSA1 Antibody (4H9-D7) Summary

• Immunogen: Human Aha1, his tagged
• Localization: Cytoplasm , Endoplasmic Reticulum
• Specificity: Detects approx 38kDa, can run up to 45kDa on SDS Page. It sees human Aha1, Mouse Aha1 and Mouse Aha2.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AHSA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:1000
• Application Notes: 1 ug/ml of AHA1 Antibody was sufficient for detection of Aha1 in 10 ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for AHSA1 Antibody (4H9-D7)

• activator of 90 kDa heat shock protein ATPase homolog 1
• AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
• C14orf3
• chromosome 14 open reading frame 3
• p38AHA1

Background

1 ug/mL was sufficient for detection of Aha1 in 10ug of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHSA1
• Uniprot:
  • NP_036243.1(Human)