Agrin Antibody [Unconjugated]

Images

 
Agrin was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Rat Agrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF550) at 0.1 µg/mL overnight at 4 °C. Tissue was stained using ...read more
Recombinant Rat Agrin (Catalog # 550-AG) induces acetylcholine receptor clustering on chick myotubes in a dose-dependent manner (orange line). Acetylcholine Receptor Clustering elicited by Recom­binant Rat Agrin ...read more

Product Details

Summary
Reactivity RtSpecies Glossary
Applications WB, IHC, B/N, ELISA(Cap)
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Agrin Antibody [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant rat Agrin
Ala1153-Pro1959 (Pro1788-Ser1798 del)
Accession # P25304
Specificity
Detects rat Agrin in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant rat (rr) GDNF, rrIL-1 alpha, and rrL-Selectin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGRN
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • Neutralization 0.001-0.004 ug/mL
  • ELISA Standard (Matched Pair)
Application Notes
ELISA Capture: Rat Agrin Antibody (Catalog # AF550)
Standard: Recombinant Rat Agrin (Catalog # 550-AG)
Reviewed Applications
Read 1 Review rated 4
using
AF550 in the following applications:

Publications
Read Publications using
AF550 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Agrin Antibody [Unconjugated]

  • agrin proteoglycan
  • Agrin
  • AGRN

Background

Agrin, a heparan sulfate proteoglycan, is a component of the synaptic basal lamina which promotes acetylcholine receptor (AChR) clustering on cultured myotubes and in vivo. This AChR clustering activity has been shown to be mediated via a receptor complex that includes a receptor-like tyrosine kinase specific to the skeletal muscle termed muscle-specific kinase (MuSK), and an as of yet unidentified myotube-specific accessory component.

Agrin contains a number of distinct domains. The N-terminal half of the molecule, which is responsible for the tight interaction with the extracellular matrix, has nine follistatin-like repeats that share homology to Kazal-type protease inhibitor domains. The C-terminal half, which by itself is sufficient for the AChR clustering activity, has four EGF-like repeats and three laminin globular G domains. Agrin exists in several isoforms which are generated by alternative splicing at multiple splicing sites in the C-terminal half. Some of these isoforms are expressed specifically in the nervous system while other isoforms are expressed in both neural and nonneural tissues. Dramatic differences in AChR clustering activities have been observed between the different isoforms. The highest activity is found to be associated with isoforms found exclusively in neural tissues that contain the four amino acids K-S-R-K at position # 1643‑1646 and the eight amino acids E-L-T-N-E-I-P-A after Ser1779. Rat and chick Agrin share approximately 60% amino acid sequence identity. R&D Systems recombinant C-terminal half fragment of Agrin is a soluble secreted protein which has been found to have AChR clustering activity on chick myotubes.

  1. Kleiman, R.J. and L.F.Reichardt (1996) Cell 85:461.
  2. Glass, D. et al. (1996) Cell 85:513.
  3. Bowe, M. and J. Fallon (1995) Annu. Rev. Neurosci. 18:443.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Agrin Antibody (AF550)(2)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: ICC, IHC Frozen.


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ICC
(1)
IHC Frozen
(1)
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(1)
Rat
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Review for Agrin Antibody (AF550) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF550:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Mouse 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 23986861
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23986861

Details

Detection NotesSee PMID 23986861

Comments

CommentsPublished: See PMID 23986861Independent review from antibodyresource.com

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Recent Reviews

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Anonymous
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol AGRN
Entrez
Uniprot