Agrin Antibody (AGR 131)

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Immunohistochemistry-Paraffin: Agrin Antibody (AGR 131) [NB110-11069] - Analysis of rat brain tissue using Agrin, mAb (Agr-131).

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, MiAr
Clone
AGR 131
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Agrin Antibody (AGR 131) Summary

Immunogen
Recombinant rat agrin (C-terminal fragment).
Localization
Synaptic basal lamina at the neuromuscular junction.
Specificity
detects all isoforms of agrin. This recognizes all N1 isoforms but, consistent with the epitope mapping investigations, fails to see the N4 protein. Furthermore, the will detect the X, Y and Z splice variants.
Isotype
IgG2 Alpha
Clonality
Monoclonal
Host
Mouse
Gene
AGRN
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Microarray
Application Notes
Detects a band of ~100 kDa by Western blot.

Reactivity Notes

Cross-reacts with Mouse and Rat. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Agrin Antibody (AGR 131)

  • agrin proteoglycan
  • Agrin
  • AGRN

Background

Agrin is an essential extracellular matrix component which promotes clustering of nicotinic acetylcholine receptors (nAChRs) and other proteins during development at the neuromuscular junction. Agrin, MuSK and Rapsyn are all essential components for AChR aggregation, through an unknown mechanism. The C-terminal region of agrin is released into the medium, interacts with receptors on the muscle surface and induces AChR aggregation. The central region contains two O-linked glycosylation sites and a domain homologous to domain III of laminin. The N-terminal region anchors agrin to the extracellular matrix via other basal membrane components. This region also contains a protease inhibitor domain and glycosaminoglycan attachment sites; increasing the predicted MW from 200kDa to ~600kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol AGRN
Entrez
Uniprot