AGO1/EIF2C1 Antibody

Images

 
Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AGO1/EIF2C1 Antibody Summary

Immunogen
Peptide with sequence C-KNASYNLDPYIQEF corresponding to internal region according to NP_036331.1.
Specificity
This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EIF2C1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: In transfected HEK293 transiently expressing human EIF2C1bands of approx. 110 kDa band and 35 kDa band are observed. The 110 kDa band is not observed in the non-transfected HEK293. The calculated molecular size is 97.2 kDa band according to NP_036331.1.
IHC: Paraffin embedded Human Placenta and Small Intestine.
Positive Control
AGO1/EIF2C1 Lysate (NBL1-10182)
Publications
Read Publications using
NB100-2817 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AGO1/EIF2C1 Antibody

  • AGO1Q99
  • argonaute 1
  • argonaute1
  • DKFZp686M13167
  • eIF2C 1
  • eIF-2C 1
  • EIF2C
  • Eukaryotic translation initiation factor 2C 1
  • eukaryotic translation initiation factor 2C, 1
  • GERP95
  • Golgi Endoplasmic Reticulum protein 95 kDa
  • hAgo1
  • protein argonaute-1
  • Putative RNA-binding protein Q99

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for AGO1/EIF2C1 Antibody (NB100-2817)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol EIF2C1
Entrez
Uniprot