Aggrecan Antibody [Unconjugated]

Aggrecan was detected in immersion fixed rat mesenchymal stem cells differentiated to chondrocytes using Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Aggrecan was detected in immersion fixed paraffin-embedded sections of human cartilage using 15 µg/mL Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

In vitro differentiation capacity of young and old BM-MSCs: (A) Representative micrographs from each experimental group showing morphological change after 21 days of differentiation. (B) Results of adipogenic, osteogenic and chondrogenic differentiation. Representative micrographs showing anti-FABP4, Oil Red O, anti-osteocalcin, Alizarin Red-S and anti-aggrecan staining in cultured BM-MSCs from each experimental group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21992089), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Aggrecan Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Aggrecan
  Val20-Arg675
  Accession # NP_037359
• Specificity: Detects human Aggrecan in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACAN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Aggrecan Antibody [Unconjugated]

• ACAN
• AGC1
• AGC1SEDK
• aggrecan core protein
• Aggrecan
• Cartilage-specific proteoglycan core protein
• Chondroitin sulfate proteoglycan 1
• Chondroitin sulfate proteoglycan core protein 1
• CSPCP
• CSPG1
• CSPG1aggrecan 1
• CSPGCP
• large aggregating proteoglycan
• MSK16
• MSK16AGCAN
• SEDK

Background

Aggrecan, also known as aggrecan 1, chondroitin sulfate proteoglycan, and large aggregating proteoglycan, is encoded by the AGC1 gene with gene aliases of SEDK; CSPG1; MSK16; CSPGCP. As the key component of the cartilage extracellular matrix, aggrecan hydrates the collagen network and provides cartilage with its properties of compressibility and elasticity. Maintenance of aggrecan content is therefore critical to the function of the tissue and aggrecan degradation is an important factor in the erosion of articular cartilage in arthritic diseases (2). The deduced amino acid sequence of human aggrecan core protein consists of 2415 resides and predicts a signal peptide and domains of G1, IGD, G2, KS, CS-1, CS-2, and G3 (3). Two globular domains, G1 and G2, comprise the N-terminus of the proteoglycan and also contain link domains. The third globular domain, G3, corresponds to the C-terminus. The keratan sulfate (KS) and the chondroitin sulfate (CS) attachment domains are between G2 and G3. With KS and CS attached to the 250 kDa core protein, aggrecan monomers exist as a 1,000-2,000 kDa molecule. In addition, aggrecan monomers interact with hyaluronan through their G1 domain, resulting in larger aggregates containing 10-100 aggrecan monomers on a hyaluronan backbone (2). Aggrecan can be cleaved by MMPs and ADAMTSs at the Asn360-Phe361 and Glu392-Ala393 bond in the IGD (residues are numbered based on Accession # NP_037359), respectively (2). Inhibition of ADATMS4 and ADAMTS5 cleavage prevents aggrecan degradation in osteoarthritic cartilage, while mice with aggrecan resistant to MMP cleavage do not accumulate aggrean and develop normally (2, 4). Consisting of the G1, IGD and G2 domains, rhAggrecan can be used as a protein substrate for MMPs and ADAMTSs and in binding assays involving hyaluronan.

1. Doege. K.J. et al. (1991) J. Biol. Chem. 266:894.
2. Malfait, A-M. et al. (2002) J. Biol. Chem. 277:22201.
3. Caterson, B. et al. (2000) Matrix Biol. 19:333.
4. Little, C.B. et al. (2005) Mol. Cel. Biol. 25:3388.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aggrecan Antibody (AF1220)(21)

Publications using AF1220

• Gomez-Sequeda, N;Mendivil-Perez, M;Jimenez-Del-Rio, M;Lopera, F;Velez-Pardo, C; Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology Scientific reports 2023-08-08 [PMID: 37553376] (ICC, Human)
• Chen, MF;Hu, CC;Hsu, YH;Chiu, YT;Chen, KL;Ueng, SWN;Chang, Y; Characterization and Advancement of an Evaluation Method for the Treatment of Spontaneous Osteoarthritis in STR/ort Mice: GRGDS Peptides as a Potential Treatment for Osteoarthritis Biomedicines 2023-04-06 [PMID: 37189729] (IHC, Mouse)
• Wu, JY;Yeager, K;Tavakol, DN;Morsink, M;Wang, B;Soni, RK;Hung, CT;Vunjak-Novakovic, G; Directed differentiation of human iPSCs into mesenchymal lineages by optogenetic control of TGF-? signaling Cell reports 2023-05-12 [PMID: 37178118] (IHC, Human)
• S Yamazaki, R Hirayama, Y Ikeda, S Iseki, T Yoda, MA Ikeda Hyaluronic acid hydrogels support to generate integrated bone formation through endochondral ossification in vivo using mesenchymal stem cells PLoS ONE, 2023-02-02;18(2):e0281345. 2023-02-02 [PMID: 36730328] (Western Blot, Human)
• Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 2022-07-15 [PMID: 35754733] (Immunohistochemistry, Human)
• Wensen Jiang, Juliane D. Glaeser, Khosrowdad Salehi, Giselle Kaneda, Pranav Mathkar, Anton Wagner et al. Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs iScience 7/15/2022 [PMID: 35754733]
• Makoto Kondo, Sumako Kameishi, Kyungsook Kim, Nicolas F. Metzler, Travis G. Maak, Douglas T. Hutchinson et al. Safety and efficacy of human juvenile chondrocyte-derived cell sheets for osteochondral defect treatment npj Regenerative Medicine 10/15/2021 [PMID: 34654830]
• A Mik?osz, B ?ukaszuk, E Supruniuk, K Grubczak, M Moniuszko, B Choroma?sk, P My?liwiec, A Chabowski Does TBC1D4 (AS160) or TBC1D1 Deficiency Affect the Expression of Fatty Acid Handling Proteins in the Adipocytes Differentiated from Human Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Obtained from Subcutaneous and Visceral Fat Depots? Cells, 2021-06-16;10(6):. 2021-06-16 [PMID: 34208471] (IHC, Human)
• C Liang, Z Liu, M Song, W Li, Z Wu, Z Wang, Q Wang, S Wang, K Yan, L Sun, T Hishida, Y Cai, JCI Belmonte, P Guillen, P Chan, Q Zhou, W Zhang, J Qu, GH Liu Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration Cell Res., 2020-07-31;0(0):. 2020-07-31 [PMID: 32737416] (ICC, Human)
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Western Blot, Mouse)
• R Lopez Pere, J Brauer, A Rühle, T Trinh, S Sisombath, P Wuchter, AL Grosu, J Debus, R Saffrich, PE Huber, NH Nicolay Human mesenchymal stem cells are resistant to UV-B irradiation Sci Rep, 2019-12-27;9(1):20000. 2019-12-27 [PMID: 31882818] (IHC-Fr, Human)
• S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (ICC, Human)
• Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis European Respiratory Journal 3/1/2019 [PMID: 30578393]
• Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis European Respiratory Journal 2019-03-01 [PMID: 30578393] (Mouse, Immunohistochemistry)
• W Srisuthtay, D Pruksakorn, P Kongtawele, P Pothacharo Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes BMC Complement Altern Med, 2017-05-31;17(1):286. 2017-05-31 [PMID: 28569157] (Western Blot, Human)
• K Tatebayash, Y Tanaka, A Nakano-Doi, R Sakuma, S Kamachi, M Shirakawa, K Uchida, H Kageyama, T Takagi, S Yoshimura, T Matsuyama, T Nakagomi Identification of multipotent stem cells in human brain tissue following stroke Stem Cells Dev, 2017-04-19;0(0):. 2017-04-19 [PMID: 28323540] (ICC, Human)
• Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC. Sci Rep, 2016-07-06;6(0):29160. 2016-07-06 [PMID: 27381039] (ICC, IHC, Human)
• Marfia G, Navone S, Di Vito C, Tabano S, Giammattei L, Di Cristofori A, Gualtierotti R, Tremolada C, Zavanone M, Caroli M, Torchia F, Miozzo M, Rampini P, Riboni L, Campanella R Gene expression profile analysis of human mesenchymal stem cells from herniated and degenerated intervertebral discs reveals different expression of osteopontin. Stem Cells Dev, 2014-10-29;24(3):320-8. 2014-10-29 [PMID: 25203751] (ICC, Human)
• Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies. PLoS ONE, 2013-01-29;8(1):e55319. 2013-01-29 [PMID: 23383153] (IHC-Fr, Human)
• Asumda FZ, Chase PB Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 2011-10-12;12(0):44. 2011-10-12 [PMID: 21992089] (ICC, Rat)
• Tautzenberger A, Lorenz S, Kreja L, Zeller A, Musyanovych A, Schrezenmeier H, Landfester K, Mailander V, Ignatius A Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation. Biomaterials, 2009-12-11;31(8):2064-71. 2009-12-11 [PMID: 20004969] (ICC, Human)

Bioinformatics

• Gene Symbol: ACAN
• Uniprot:
  • NP_037359()