AGAP3 Antibody

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Immunohistochemistry-Paraffin: AGAP3 Antibody [NB100-1323] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

AGAP3 Antibody Summary

Immunogen
Peptide with sequence C-NPSAELHRSPSLL corresponding to C-Terminus according to NP_114152.3.
Predicted Species
Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
AGAP3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 45 kDa band in human brain and human liver lysates at 1 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 101 kDa according to NP_114152. IHC-P: Human kidney shows strong staining of brush borders in proximal convoluted tubules.
Publications
Read Publication using NB100-1323.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for AGAP3 Antibody

  • ANK repeat and PH domain-containing protein 3
  • ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
  • centaurin-gamma-3
  • cnt-g3
  • CRAG
  • CRAM-associated GTPase
  • CRMP (collapsin response mediator protein) associated
  • FLJ16146
  • FLJ34452
  • gamma 3
  • MR1-interacting protein
  • MRIP-1

Background

CENTG3, or M-RIP, is a 116 kDa protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-1323
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Applications: IHC, IHC-P, PEP-ELISA

Publications for AGAP3 Antibody (NB100-1323)(1)

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Secondary Antibodies

 

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Array NB100-1323

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Bioinformatics

Gene Symbol AGAP3
Entrez
Uniprot