AGAP3 Antibody

Immunohistochemistry-Paraffin: AGAP3 Antibody [NB100-1323] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AGAP3 Antibody Summary

• Description: Novus Biologicals Goat AGAP3 Antibody (NB100-1323) is a polyclonal antibody validated for use in IHC and ELISA. Anti-AGAP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NPSAELHRSPSLL corresponding to C-Terminus according to NP_114152.3.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AGAP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Preliminary experiments gave an approx. 45 kDa band in human brain and human liver lysates at 1 ug/ml, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in scientific literature for the band we observe given the predicted MW of approx. 101 kDa according to NP_114152. IHC-P: Human kidney shows strong staining of brush borders in proximal convoluted tubules.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AGAP3 Antibody

• ANK repeat and PH domain-containing protein 3
• ArfGAP with GTPase domain, ankyrin repeat and PH domain 3
• centaurin-gamma-3
• cnt-g3
• CRAG
• CRAM-associated GTPase
• CRMP (collapsin response mediator protein) associated
• FLJ16146
• FLJ34452
• gamma 3
• MR1-interacting protein
• MRIP-1

Background

CENTG3, or M-RIP, is a 116 kDa protein that interacts with myosin phosphatase and RhoA in vascular smooth muscle cells. Because CENTG3 is able to assemble a complex containing both RhoA and MBS, it is speculated that this protein may play a role in myosin phosphatase regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AGAP3 Antibody (NB100-1323)(1)

Publication using NB100-1323

• Surks HK, Richards CT, Mendelsohn ME. Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA. J Biol Chem 2003-12-19 [PMID: 14506264]

Bioinformatics

• Gene Symbol: AGAP3
• Entrez:
  • 116988(Human)
  • 213990(Mouse)
• Uniprot:
  • NP_114152.(Human)