Afamin Antibody [Alexa Fluor® 350]

• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Afamin Antibody [Alexa Fluor® 350] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived recombinant human Afamin
  Leu22-Asn599
  Accession # P43652
• Specificity: Detects human Afamin in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse Afamin is observed, and less than 1% cross-reactivity with recombinant human (rh) AFB and rhAFP is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

AFM (Afamin; also alpha -Albumin) is a secreted monomeric glycoprotein member of the Alb/albumin family of molecules. Although its MW in SDS-Page ranges from 84-88 kDa, MALDI-TOF analysis yields a likely more correct value of 70-75 kDa. It is expressed by hepatocytes, CNS endothelial cells and osteoclasts, and circulates in the blood at low μg/mL concentrations. AFM is known to bind and transport vitamin E, particularly under conditions where lipoprotein is limited. This is likely to be important in follicular fluid and CSF. It also serves as an osteoclast-derived chemoattractant for preosteoblasts, providing a rational for the observation that bone formation often follows bone resorption. Mature human AFM is 578 amino acids (aa) in length (aa 22-599). It contains three consecutive albumin domains (aa 36-206, 211-403 and 404-599) that contain a characteristic 5 or 6 intrachain disulfide bonds. Full-length human AFM shares 67% aa sequence identity with mouse AFM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.