Human Adropin ELISA Kit (Colorimetric)

ELISA: Human Adropin ELISA Kit (Colorimetric) [NBP2-66433] - Samples were spiked with high concentrations of Human Adropin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Adropin ELISA Kit (Colorimetric) [NBP2-66433] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 12.50 - 800 pg/mL
• Sensitivity: 7.50 pg/mL

Human Adropin ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human AD in samples. No significant cross-reactivity or interference between Human AD and analogues was observed.
• Standard Curve Range: 12.50 - 800 pg/mL
• Sensitivity: 7.50 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.18%
• Intra-Assay: CV% < 5.24%
• Spike Recovery: 89-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ENHO

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Adropin ELISA Kit (Colorimetric)

• Adropin
• C9orf165
• chromosome 9 open reading frame 165
• energy homeostasis associated
• Energy homeostasis-associated protein
• ENHO
• GAAI470
• UNQ470

Background

Energy homeostasis-associated protein (ENHO), also known as Adropin, helps to ] regulate glucose homestasis and lipid metabolism. Adropin is also thought to affect the activity of other metabolic genes, specifically those involved in the production of lipids from carbohydrates.

Adropin is a secreted protein encoded by the ENHO gene found in the brain and liver. In the liver, Adropin has been found to increase in response to high-fat foods and decrease after fasting.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Adropin ELISA Kit (NBP2-66433)(2)

Publications using NBP2-66433

• Dodd WS, Patel D, Lucke-Wold B Et al. Adropin decreases endothelial monolayer permeability after cell-free hemoglobin exposure and reduces MCP-1-induced macrophage transmigration Biochemical and biophysical research communications 2021-10-16 [PMID: 34710824] (ELISA)
• Chen X, Sun X, Shen T et al. Lower adropin expression is associated with oxidative stress and severity of nonalcoholic fatty liver disease Free Radic. Biol. Med. 2020-08-15 [PMID: 32810635]

FAQs for Adropin ELISA Kit (NBP2-66433). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: ENHO