Adrenomedullin/ADM Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC200507 and based on accession number NM_001124.
Homo sapiens adrenomedullin (ADM), mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Adrenomedullin/ADM
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Adrenomedullin/ADM Overexpression Lysate (Native)
Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Rt, Po, Bv, Ca, Eq, Gt, Ha, Mk, Pm, Rb, Xp
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
Applications: WB, Flow, IHC, CyTOF-ready
Species: Hu, Mk
Applications: WB, Flow, IHC, IHC-P
Applications: WB, IHC
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Applications: ICC/IF, IHC, IHC-P
Applications: ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Ca, Pm
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, IHC-P, IP
Applications: WB, IHC, IP, Neut
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Publications for Adrenomedullin/ADM Lysate (NBL1-07345) (0)
There are no publications for Adrenomedullin/ADM Lysate (NBL1-07345).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Adrenomedullin/ADM Lysate (NBL1-07345) (0)
There are no reviews for Adrenomedullin/ADM Lysate (NBL1-07345).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
- 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for Adrenomedullin/ADM Lysate (NBL1-07345). (Showing 1 - 2 of 2 FAQ).
I have got a query regarding the following products: catalog # NBL1-07345 and catalog # H00000133-T04. Are they the same product/source?
- NBL1-07345 and H00000133-T04 are both Adrenomedullin Overexpression Lysates but they are not the exact same products. H00000133-T04 is an Abnova product and NBL1-07345 is a Novus product. The main difference between these two is NBL1-07345 contains DDK/Myc tag at C-Terminus so you can use both Adrenomedullin and DDK antibody to detect the protein.
We are interested in the following references: NBL1-11754, NB820-59468, NBL1-14127,NBL1-07345. Have these references got any tag in their sequence for their purification, so the weight (KDa) increases?
- NBL1-11754, NBL1-14127 and NBL1-07345 are all overexpression lysates; in each case, the protein contains a C-terminal Myc/DDK epitope tag (only 3 kDa total). The NB820-59468 is not an overexpression lysate; it is a whole normal brain (fetal) tissue lysate.
Additional Adrenomedullin/ADM Products
Bioinformatics Tool for Adrenomedullin/ADM Lysate (NBL1-07345)
Discover related pathways, diseases and genes to Adrenomedullin/ADM Lysate (NBL1-07345). Need help?
Read the Bioinformatics Tool Guide
for instructions on using this tool.
Blogs on Adrenomedullin/ADM