Adrenomedullin/ADM Overexpression Lysate (Native)


Western Blot: Adrenomedullin Overexpression Lysate (Adult Normal) [NBL1-07345] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Adrenomedullin.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

Adrenomedullin/ADM Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC200507 and based on accession number NM_001124.
C-terminal Myc/DDK
Homo sapiens adrenomedullin (ADM), mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for Adrenomedullin/ADM

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Adrenomedullin/ADM Overexpression Lysate (Native)

  • ADM
  • adrenomedullin
  • AMpreproadrenomedullin


Adrenomedullin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomedullin, is 185 amino acids long. By RNA-blot analysis, human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns, with the 5-prime flanking region containing TATA, CAAT, and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Adrenomedullin/ADM Lysate (NBL1-07345) (0)

There are no publications for Adrenomedullin/ADM Lysate (NBL1-07345).
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Adrenomedullin/ADM Lysate (NBL1-07345). (Showing 1 - 2 of 2 FAQ).

  1. I have got a query regarding the following products: catalog # NBL1-07345 and catalog # H00000133-T04. Are they the same product/source?
    • NBL1-07345 and H00000133-T04 are both Adrenomedullin Overexpression Lysates but they are not the exact same products. H00000133-T04 is an Abnova product and NBL1-07345 is a Novus product. The main difference between these two is NBL1-07345 contains DDK/Myc tag at C-Terminus so you can use both Adrenomedullin and DDK antibody to detect the protein.
  2. We are interested in the following references: NBL1-11754, NB820-59468, NBL1-14127,NBL1-07345. Have these references got any tag in their sequence for their purification, so the weight (KDa) increases?
    • NBL1-11754, NBL1-14127 and NBL1-07345 are all overexpression lysates; in each case, the protein contains a C-terminal Myc/DDK epitope tag (only 3 kDa total). The NB820-59468 is not an overexpression lysate; it is a whole normal brain (fetal) tissue lysate.

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Contact Information

Product PDFs


Gene Symbol ADM

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