Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

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Canine Adrenomedullin/ADM ELISA Kit (Colorimetric) [NBP2-77008] - Standard Curve Reference

Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and cell culture supernates
Standard Curve Range
15.6 pg/mL - 1000 pg/mL
Sensitivity
3.9 pg/mL

Order Details

Canine Adrenomedullin/ADM ELISA Kit (Colorimetric) Summary

Description
Assay Length: 1-5 hours
Specificity
This assay has high sensitivity and excellent specificity for detection of Canine Adrenomedullin/ADM. No significant cross-reactivity or interference between Canine Adrenomedullin/ADM and analogues was observed.
Standard Curve Range
15.6 pg/mL - 1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 10
Intra-Assay
%CV < 8
Spike Recovery
82 - 110%
Sample Volume
50 - 100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
ADM

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Strip (For 96 wells)
  2. Assay Plate (12 x 8 coated Microwells)
  3. Biotin-antibody (100 x concentrate)
  4. Biotin-antibody Diluent
  5. HRP-avidin (100 x concentrate)
  6. HRP-avidin Diluent
  7. Instruction Manual
  8. Sample Diluent
  9. Standard (Freeze dried)
  10. Stop Solution
  11. TMB Substrate
  12. Wash Buffer (25 x concentrate)

Alternate Names for Canine Adrenomedullin/ADM ELISA Kit (Colorimetric)

  • ADM
  • Adrenomedullin
  • AMpreproadrenomedullin

Background

Adrenomedullin is a member of the calcitonin family of peptides and functions as a vasodilator. Adrenomedullin, a hypotensive peptide, consists of 52 amino acids, has 1 intramolecular disulfide bond, and shows a slight homology with the calcitonin gene related peptide. Human adrenomedullin mRNA was found to be highly expressed in several tissues. Adrenomedullain is secreted from a number of tissues including the adrenal medulla, endothelial and vascular smooth muscle cells. It has been implicated in a number of diseases including cardiovascular and renal disorders, sepsis, inflammation, diabetes and cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol ADM