ADP-Sugar Pyrophosphatase/NUDT5 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

ADP-Sugar Pyrophosphatase/NUDT5 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human ADP‑Sugar Pyrophosphatase/NUDT5
  Glu2-Phe219
  Accession # Q9UKK9
• Specificity: Detects human ADP‑Sugar Pyrophosphatase/NUDT5 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADP-Sugar Pyrophosphatase/NUDT5 Antibody [Alexa Fluor® 405]

• EC 3.6.1.-
• EC 3.6.1.13
• hYSAH1
• nucleoside diphosphate linked moiety X-type motif 5
• Nucleoside diphosphate-linked moiety X motif 5
• nudix (nucleoside diphosphate linked moiety X)-type motif 5
• Nudix motif 5
• NUDT5
• YSA1
• YSA1H
• YSA1HADP-sugar pyrophosphatase

Background

ADP‑Sugar Pyrophosphatase (NUDT-5, Nucleoside diphosphate-linked moiety X motif 5; also YSA1H and 8-oxo-dGDP phosphatase) is a member of the Nudix hydrolase family of proteins. Although the predicted MW of NUDT-5 is 24 kDa, it runs anomalously at 32-36 kDa in SDS-Page. NUDT-5 is a widely expressed intracellular homodimer that has at least two functions. First, it possesses pyrophosphatase activity that prevents nonenzymatic ribosylation of proteins, and second, it hydrolyzes 8-oxoGDP (plus 8-OH-dADP), thus removing an oxidized nucleoside from the nucleotide pool and its potential for incorporation into either DNA or RNA. Such incorporation likely results in transcriptional or translational errors. Human NUDT-5 is 219 amino acids (aa) in length (SwissProt #:Q9UKK9). It is contains an N-terminal substrate binding region (aa 28-51), a Nudix hydrolase domain (aa 57-197) and an extended C-terminal a-helix (aa 211-219) NUDT-5 is acetylated on Met1, Lys42, Lys210, and Lys218, and phosphorylated on Tyr74. There are two isoform variants, one that shows a deletion of aa 163-165, and another that contains a 13 aa substitution for aa 164-219. Full-length human and mouse NUDT-5 share 82% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.