ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, and human liver tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human ADP-Sugar Pyrophosphatase/NUDT5 Monoclonal Antibody (Catalog # MAB6414) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADP-Sugar Pyrophosphatase/NUDT5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 739044
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044) Summary

• Immunogen: E. coli-derived recombinant human ADP-Sugar Pyrophosphatase/NUDT5
  Glu2-Phe219
  Accession # Q9UKK9
• Specificity: Detects human ADP-Sugar Pyrophosphatase/NUDT5 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUDT5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADP-Sugar Pyrophosphatase/NUDT5 Antibody (739044)

• EC 3.6.1.-
• EC 3.6.1.13
• hYSAH1
• nucleoside diphosphate linked moiety X-type motif 5
• Nucleoside diphosphate-linked moiety X motif 5
• nudix (nucleoside diphosphate linked moiety X)-type motif 5
• Nudix motif 5
• NUDT5
• YSA1
• YSA1H
• YSA1HADP-sugar pyrophosphatase

Background

ADP-Sugar Pyrophosphatase (NUDT5) is a member of the nudix superfamily of enzymes. Members of this family are pyrophosphohydrolases that act upon substrates with the general structure of a nucleoside (Nu) diphosphate (di) linked to another moiety, X (NDP-X) to yield NMP plus P-X (1). Human NUDT5 is a homodimeric enzyme present in the cytosol of most cell types (2). Glu166 and three magnesium ions are important for stabilizing the transition state during the hydrolysis of ADPR (3). NUDT5 has been suggested to play a role in regulating the intracellular levels of ADPR by NO activation through ADP-ribosylation at cysteine residues of the enzyme in macrophages (4). It also may play defensive role against the mutagenesis induced by oxidized deoxyribonucleosides (5, 6).

1. McLennan, A.G.  (2006) Cell Mol. Life Sci. 63:123.
2. Zha, M. et al. (2006) J. Mol. Biol. 364:1021.
3. Zha, M. et al. (2008) J. Mol. Biol. 379:568.
4. Yu, H.N. et al. (2007) Biochem. Biophys. Res. Comm. 354:764.
5. Hori, M. et al. (2010) Free Radic. Biol. Med. 48:1197.
6. Kamiya, H. et al. (2009) DNA Repair (Amst) 8:1250.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUDT5
• Uniprot:
  • Q9UKK9()