Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

ELISA: Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric) [NBP2-68181] - Samples were spiked with high concentrations of Mouse Adenylate Kinase 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric) [NBP2-68181] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 mIU/mL
• Sensitivity: 0.47 mIU/mL

Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse AK1 in samples. No significant cross-reactivity or interference between Mouse AK1 and analogues was observed.
• Standard Curve Range: 0.78 - 50 mIU/mL
• Sensitivity: 0.47 mIU/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.87%
• Intra-Assay: CV% < 5.28%
• Spike Recovery: 84-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: AK1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Adenylate Kinase 1 ELISA Kit (Colorimetric)

• Adenylate Kinase 1
• adenylate kinase isoenzyme 1
• AK 1
• AK1
• ATP-AMP transphosphorylase 1
• EC 2.7.4
• EC 2.7.4.3
• EC 2.7.4.6
• Myokinase

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: AK1