Adenylate Kinase 1 Antibody (3A6-1F5) - Azide and BSA Free

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of AK1 expression in transfected 293T cell line by AK1 monoclonal antibody (M06), clone 3A6-1F5. Lane 1: AK1 transfected lysatE (21.6 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of monoclonal antibody to AK1 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Western blot analysis of AK1 over-expressed 293 cell line. Lane 2 cotransfected with AK1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000203-M06. GAPDH ( 36.1 kDa ) used as loading control.

Western Blot: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of AK1 expression in HeLa (Cat # L013V1).

Immunoprecipitation: Adenylate Kinase 1 Antibody (3A6-1F5) [H00000203-M06] - Analysis of AK1 transfected lysate using anti-AK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AK1 rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IP, KD
• Clone: 3A6-1F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Adenylate Kinase 1 Antibody (3A6-1F5) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AK1 (AAH01116, 1 a.a. ~ 194 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEEKLKKTNIIFVVGGPGSGKGTQCEKIVQKYGYTHLSTGDLLRSEVSSGSARGKKLSEIMEKGQLVPLETVLDMLRDAMVAKVNTSKGFLIDGYPREVQQGEEFERRIGQPTLLLYVDAGPETMTQRLLKRGETSGRVDDNEETIKKRLETYYKATEPVIAFYEKRGIVRKVNAEGSVDSVFSQVCTHLDALK
• Specificity: AK1 - adenylate kinase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AK1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein and cell lysate for WB. It has also been used for IF, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Adenylate Kinase 1 Antibody (3A6-1F5) - Azide and BSA Free

• Adenylate Kinase 1
• adenylate kinase isoenzyme 1
• AK 1
• AK1
• ATP-AMP transphosphorylase 1
• EC 2.7.4
• EC 2.7.4.3
• EC 2.7.4.6
• Myokinase

Background

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate group among adinine nucleotides. Three isozymes of adenylate kinase have been identified in vertebrates, adenylate isozyme 1 (AK1), 2 (AK2) and 3 (AK3). AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. AK1 was identified because of its association with a rare genetic disorder causing nonspherocytic hemolytic anemia where a mutation in the AK1 gene was found to reduce the catalytic activity of the enzyme.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AK1
• Entrez:
  • 203(Human)
• OMIM:
  • 103000(Human)
• Uniprot:
  • AAH01116(Human)