Adenosylhomocysteinase/AHCY Antibody [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

Adenosylhomocysteinase/AHCY Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human Adenosylhomocysteinase/AHCY
  Ser2-Tyr432
  Accession # P23526
• Specificity: Detects human Adenosylhomocysteinase/AHCY in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Adenosylhomocysteinase/AHCY Antibody [Alexa Fluor® 647]

• Adenosylhomocysteinase
• AdoHcyase
• AHCY
• EC 3.3.1.1
• S-adenosylhomocysteine hydrolase
• S-adenosyl-L-homocysteine hydrolase
• SAHH
• SAHHadoHcyase

Background

Human S‑Adenosylhomocysteinase (AHCY) is a cytoplasmic tetramer with a tightly bound NAD co‑factor for each subunit (1, 2). It is the only known enzyme to catalyze the breakdown of S‑adenosylhomocysteine (AdoHcy) to homocysteine and adenosine. AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCY’s activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease (3). It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S‑adonosyl-L-methionine methyltransferases (1). A mutation in the human AHCY results in AHCY deficiency with increase of plasma creatine kinase, methionine, S‑adenosylmethionine and AdoHcy, delay of myelination, myopathy and psychomotor retardation (4, 5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.