Adenosylhomocysteinase/AHCY Antibody (4H2)

Western Blot: Adenosylhomocysteinase/AHCY Antibody (4H2) [H00000191-M07A] - Analysis of AHCY expression in A-549 (Cat # L025V1).

Product Discontinued

Adenosylhomocysteinase/AHCY Antibody (4H2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: AHCY (AAH11606, 1 a.a. ~ 432 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDKLPYKVADIGLAAWGRKALDIAENEMPGLMRMRERYSASKPLKGARIAGCLHMTVETAVLIETLVTLGAEVQWSSCNIFSTQDHAAAAIAKAGIPVYAWKGETDEEYLWCIEQTLYFKDGPLNMILDDGGDLTNLIHTKHPQLLPGIRGISEETTTGVHNLYKMMANGILKVPAINVNDSVTKSKFDNLYGCRESLIDGIKRATDVMIAGKVAVVAGYGDVGKGCAQALRGFGARVIITEIDPINALQAAMEGYEVTTMDEACQEGNIFVTTTGCIDIILGRHFEQMKDDAIVCNIGHFDVEIDVKWLNENAVEKVNIKPQVDRYRLKNGRRIILLAEGRLVNLGCAMGHPSFVMSNSFTNQVMAQIELWTHPDKYPVGVHFLPKKLDEAVAEAHLGKLNVKLTKLTEKQAQYLGMSCDGPFKPDHYRY
• Specificity: AHCY - S-adenosylhomocysteine hydrolase (4H2)
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: AHCY
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: No Preservative
• Purity: Unpurified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Adenosylhomocysteinase/AHCY Antibody (4H2)

• Adenosylhomocysteinase
• AdoHcyase
• AHCY
• EC 3.3.1.1
• S-adenosylhomocysteine hydrolase
• S-adenosyl-L-homocysteine hydrolase
• SAHH
• SAHHadoHcyase

Background

S-adenosylhomocysteine hydrolase catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy). Thus, it regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. S-adenosylhomocysteine hydrolase belongs to the adenosylhomocysteinase family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Adenosylhomocysteinase/AHCY Antibody (H00000191-M07A)(3)

Publications using H00000191-M07A

• Lee HO, Wang L, Kuo YM et al. S-adenosylhomocysteine hydrolase over-expression does not alter S-adenosylmethionine or S-adenosylhomocysteine levels in CBS deficient mice. Mol Genet Metab Rep 2018-06-01 [PMID: 30023284]
• Chayka O. Identification and pharmacological inactivation of the MYCN gene network as a therapeutic strategy for neuroblastic tumour cells. J Biol Chem. 2013-05-17 [PMID: 25477524]
• Fernandez-Sanchez, ME, Gonatopoulos-Pournatzis, T, Preston, G, et al. S-adenosyl homocysteine hydrolase is required for Myc-induced mRNA cap methylation, protein synthesis, and cell proliferation. Mol Cell Biol. 2009-10-05 [PMID: 19805518]

Bioinformatics

• Gene Symbol: AHCY
• Entrez:
  • 191(Human)
• Uniprot:
  • AAH11606(Human)