Recombinant Human Adenosine Deaminase/ADA Protein

Images

 
SDS-Page: Recombinant Human Adenosine Deaminase/ADA Protein [NBP1-72362]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Adenosine Deaminase/ADA Protein Summary

Description
A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-363 of Human Adenosine Deaminase/ADA

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL

Details of Functionality
Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
ADA
Purity
>85%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>85%, by SDS-PAGE

Alternate Names for Recombinant Human Adenosine Deaminase/ADA Protein

  • ADA
  • ADA1
  • adenine deaminase
  • Adenosine aminohydrolase
  • Adenosine Deaminase
  • EC 3.5.4.4

Background

ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBL1-07321
Species: Hu
AF6486
Species: Hu
Applications: WB, Simple Western
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF1180
Species: Hu
Applications: WB, Simple Western, IHC, ICC
MAB6240
Species: Hu
Applications: WB, IHC
NBP2-15291
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB600-935
Species: Bv
Applications: WB, ELISA, IP
NBP1-89238
Species: Hu
Applications: WB, Simple Western, IHC, IHC-P
NB100-1793
Species: Hu, Mu
Applications: WB, IHC, IHC-Fr, IHC-P, PEP-ELISA
NBP2-57507
Species: Hu
Applications: ICC/IF
AF6466
Species: Hu
Applications: WB
NB100-230
Species: Hu
Applications: WB, IP, PLA
NBP1-89342
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P
NBP2-45570
Species: Hu
Applications: WB, Flow, IHC
NB100-233
Species: Hu
Applications: WB, PLA

Publications for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362) (0)

There are no publications for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362) (0)

There are no reviews for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Adenosine Deaminase/ADA Products

Bioinformatics Tool for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362)

Discover related pathways, diseases and genes to Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362)

Discover more about diseases related to Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362).
 

Pathways for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362)

View related products by pathway.

PTMs for Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362)

Learn more about PTMs related to Adenosine Deaminase/ADA Recombinant Protein (NBP1-72362).

Blogs on Adenosine Deaminase/ADA

There are no specific blogs for Adenosine Deaminase/ADA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Adenosine Deaminase/ADA Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol ADA
Entrez