Recombinant Human Adenosine Deaminase/ADA Protein (NBP1-72362)

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SDS-Page: Recombinant Human Adenosine Deaminase/ADA Protein [NBP1-72362]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Adenosine Deaminase/ADA Protein Summary

Description
A recombinant protein with a His-tag corresponding to amino acids 1 to 363 of Human Adenosine Deaminase/ADA

Source: E. coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL
Preparation
Method
E.coli
Details of Functionality
Specific activity: approximately >25 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme that deaminates 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C
Protein/Peptide Type
Biologically Active Protein
Gene
ADA

Applications/Dilutions

Application Notes
Assay
1. Prerapre a 1.5 ml reaction mix: the final concentrations are 53.3mM potassium phosphate, 0.045mM adenosine, 0.003% (w/v) bovine serum.
2. Add recombinant ADA protein with various concentrations (0.1ug, 0.2) in assay buffer.
3. Mix by inversion and record A260nm for approximately 5 minutes.
Theoretical MW
42.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml

Notes

The purity of this protein is > 85% by SDS-PAGE. Molecular weight is 42.9 kDa (383aa), confirmed by MALDI-TOF

Alternate Names for Recombinant Human Adenosine Deaminase/ADA Protein

  • ADA
  • ADA1
  • adenine deaminase
  • Adenosine aminohydrolase
  • Adenosine Deaminase
  • EC 3.5.4.4

Background

ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Bioinformatics

Gene Symbol ADA
Entrez