Human Adenosine Deaminase/ADA ELISA Kit (Chemiluminescence)

ELISA: Human Adenosine Deaminase/ADA ELISA Kit (Chemiluminescence) [NBP2-66434] - Samples were spiked with high concentrations of Human Adenosine Deaminase/ADA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Adenosine Deaminase/ADA ELISA Kit (Chemiluminescence) [NBP2-66434] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL

Human Adenosine Deaminase/ADA ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human ADA in samples. No significant cross-reactivity or interference between Human ADA and analogues was observed.
• Standard Curve Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 10.59%
• Intra-Assay: CV% < 11.47%
• Spike Recovery: 93-114%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: ADA

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Adenosine Deaminase/ADA ELISA Kit (Chemiluminescence)

• ADA
• ADA1
• adenine deaminase
• Adenosine aminohydrolase
• Adenosine Deaminase
• EC 3.5.4.4

Background

Adenosine deaminase is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and from the turnover of nucleic acids in tissues. It irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the removal of an amine group. Inosine can then be deribosylated (removed from ribose) by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine. Mutations in the gene for adenosine deaminase causing it to not be expressed are one cause of severe combined immunodeficiency (SCID). Mutations causing it to be overexpressed are one cause of hemolytic anemia. There is some evidence that a different allelle (ADA2) may lead to autism. There are 2 isoforms of ADA: ADA1 and ADA2. ADA1 is found in most body cells, particularly lymphocytes and macrophages, where it is present not only in the cytosol but also as the ecto- form on the cell membrane attached to a protein called CD26. ADA2 has only been found in the macrophage where it co-exists with ADA1 where the two isoforms regulate the ratio of adenosine to deoxyadenosine to potentiate the killing of parasites. ADA2 is the predominant form present in human plasma and is increased in many diseases, particularly those associated with the immune system: for example rheumatoid arthritis, psoriasis and sarcoidosis. The plasma AD2 isoform is also increased in most cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ADA