ADAR Antibody (CL0176) - Azide and BSA Free

Staining of A549 cells using the Anti-ADAR monoclonal antibody) .

Analysis in human cerebral cortex and skeletal muscle tissues using NBP3-44046 antibody. Corresponding ADAR RNA-seq data are presented for the same tissues.

Staining of human kidney shows strong nuclear positivity in cells in tubules.

Staining of human cerebral cortex shows strong nuclear positivity in neurons.

Staining of human skeletal muscle shows no positivity in myocytes as expected.

Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ADAR antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL0176
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

ADAR Antibody (CL0176) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse ADAR Antibody (CL0176) - Azide and BSA Free (NBP3-05500) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P55265, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAR
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF fixation permeabilization use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for ADAR Antibody (CL0176) - Azide and BSA Free

• ADAR
• ADAR1
• ADAR1DSH
• adenosine deaminase, RNA-specific
• DRADA
• DRADAP136
• DSRAD
• DSRADadenosine deaminase acting on RNA 1-A
• EC 3.5.4
• EC 3.5.4.-
• EC 3.5.4.37
• G1P1
• G1P1double-stranded RNA-specific adenosine deaminase
• IFI4
• IFI-4
• IFI4dsRNA adenosine deaminase
• interferon-induced protein 4
• Interferon-inducible protein 4
• K88DSRBP
• p136
• p136,136 kDa double-stranded RNA-binding protein

Background

ADAR encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAR