Human ADAMTS5 ELISA Kit (Colorimetric)

ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Samples were spiked with high concentrations of Human ADAMTS5 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) [NBP2-66444] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL

Human ADAMTS5 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human ADAMTS5 in samples. No significant cross-reactivity or interference between Human ADAMTS5 and analogues was observed.
• Standard Curve Range: 0.78 - 50 ng/mL
• Sensitivity: 0.47 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.09%
• Intra-Assay: CV% < 5.09%
• Spike Recovery: 86-110%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: ADAMTS5

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human ADAMTS5 ELISA Kit (Colorimetric)

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

ADAMTS5, also known as aggrecanase-2, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan. The protein contains a signal sequence, a prodomain with both a potential cysteine switch and a furin cleavage site, a metalloproteinase domain with a conserved zinc-binding motif and a 'met turn,' a disintegrin-like domain, and a spacer region between a thrombospondin type 1 motif and thrombospondin submotif. At least four transcript variants have been reported. ADAMTS5 has been implicated in inflammatory joint diseases. ADAMTS5 expression has been documented in a range of normal human tissues. ESTs have been isolated from human tissue libraries, including normal breast, embryo and heart and cancerous placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ADAMTS5