ADAMTS5 Antibody

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7768] - Anti-ADAMTS5 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7768] - IHC analysis of Cartilage using this antibody at 2 ug/ ml.

Immunohistochemistry-Paraffin: ADAMTS5 Antibody [NLS7768] - Analysis of anti-ADAMTS5 antibody with human breast, carcinoma.

• Reactivity: Hu, Pm, Pm, Mu, Rt, Po, Bt, Ca, Ch, Eq, Ha, Ma-Op, Tr
• Applications: IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

ADAMTS5 Antibody Summary

• Immunogen: Synthetic 16 amino acid peptide from C-terminus of human ADAMTS5.
• Specificity: Human ADAMTS5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Mouse (94%), Rat (94%), Porcine (94%), Opossum (94%), Hamster (94%), Chicken (94%), Canine (94%), Bat (94%), Turkey (94%), Equine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ADAMTS5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
• Control:

  ADAMTS5 Overexpression Lysate

Reactivity Notes

Gorilla, Gibbon, Marmoset. Predicted cross-reactivity based on sequence identity: Panda (100%), Lizard (100%), Elephant (94%), Bovine (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ADAMTS5 Antibody

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

ADAMTS5, also known as aggrecanase-2, is a Metallopeptidase M12B type protease that is responsible for degradation of the articular cartilage protein aggrecan. The protein contains a signal sequence, a prodomain with both a potential cysteine switch and a furin cleavage site, a metalloproteinase domain with a conserved zinc-binding motif and a 'met turn,' a disintegrin-like domain, and a spacer region between a thrombospondin type 1 motif and thrombospondin submotif. At least four transcript variants have been reported. ADAMTS5 has been implicated in inflammatory joint diseases. ADAMTS5 expression has been documented in a range of normal human tissues. ESTs have been isolated from human tissue libraries, including normal breast, embryo and heart and cancerous placenta and uterus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAMTS5
• Entrez:
  • 11096(Human)