ADAMTS5 Antibody (362810) [Alexa Fluor™ Plus 594]

• Clone: 362810
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

ADAMTS5 Antibody (362810) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human ADAMTS5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ADAMTS1 or rhADAMTSL1.2 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS5 Antibody (362810) [Alexa Fluor™ Plus 594]

• A disintegrin and metalloproteinase with thrombospondin motifs 11
• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2)
• ADAM metallopeptidase with thrombospondin type 1 motif, 5
• ADAM-TS 11
• ADAM-TS 5
• ADAMTS11
• ADAMTS-11
• ADAMTS5
• ADAM-TS5
• ADAMTS-5
• ADMP2
• ADMP-2
• ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5
• Aggrecanase 2
• aggrecanase-2
• disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738
• EC 3.4.24
• EC 3.4.24.-
• EC 3.4.24.14
• EC 3.4.24.82

Background

ADAMTS5 (a disintegrin and metalloproteinase with thrombospondin motifs 5), also known as Aggrecanase-2 and ADAMTS11, is a member of the family of secreted zinc proteases with a multi-domain structure (1, 2). The protein precursors consist of signal peptide and following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, spacer and a variable number of TS type 1 motifs. ADAMTS5 is an active protease effectively cleaving alpha 2-Macroglobulin (3), Aggrecan (4), and Brevican (5), and is inhibited by TIMP-3 with inhibition constants in the subnanomolar range (6). Based on the murine model studies (7, 8), this protease may be a key enzyme in the degradation of cartilage leading to osteoarthritis and rheumatoid arthritis. The purified recombinant human (rh) ADAMTS5 starts at the N-terminus of the catalytic domain and ends at the C-terminus of the TSP-1 domain. The amino acid sequence of rhADAMTS5 is 98%, 97%, and 96% identical to that of canine, bovine, and mouse/rat. The aggrecanase activity can be inhibited by 5 mM 1,10-phenanthroline and rhTIMP-3 (R&D Systems, Catalog # 973-TM).

1. Porter, S. et al. (2005) Biochem. J. 386:15.
2. Nagase, H. and M. Kashiwagi (2003) Arthritis Res. Ther. 5:94.
3. Tortorella, M.D. et al. (2004) J. Biol. Chem. 279:17554.
4. Vankemmelbeke, M.N. et al. (2001) Eur. J. Biochem. 268:1259.
5. Nakada, M. et al. (2005) Acta Neurophathol (Berl). 110:239.
6. Kashiwagi, M. et al. (2001) J. Biol. Chem. 276:12501.
7. Glasson, S.S. et al. (2005) Nature, 434: 644.
8. Stanton, H. et al. (2005) Nature, 434:648.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.