ADAMTS4 Antibody (416608) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: ELISA
• Clone: 416608
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

ADAMTS4 Antibody (416608) [Alexa Fluor® 532] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAMTS4
  Phe213-Cys685
  Accession # O75173
• Specificity: Detects human ADAMTS4 in direct ELISA.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ADAMTS4 Antibody (416608) [Alexa Fluor® 532]

• a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 4
• ADAM metallopeptidase with thrombospondin type 1 motif, 4
• ADAM-TS 4
• ADAMTS-2
• ADAMTS4
• ADAM-TS4
• ADAMTS-4
• ADMP-1
• ADMP-1EC 3.4.24.82
• Aggrecanase 1
• aggrecanase-1
• EC 3.4.24
• KIAA0688A disintegrin and metalloproteinase with thrombospondin motifs 4

Background

ADAMTS4 (a disintegrin and metalloproteinase with thrombospondin motifs 4), also known as aggrecanase-1, is a member of the family of secreted zinc proteases with a multi-domain structure (1‑3). The protein precursors consist of a signal peptide and the following domains: pro, catalytic, disintegrin-like, TS type 1 motif, cysteine-rich, and spacer. It is the only ADAMTS identified that has one TS type I motif. It is an active protease effectively cleaving alpha -2-macroglobulin and aggrecan at multiple sites, and is inhibited by TIMP-3 with inhibition constants in subnanomolar range (4‑6). It receives great attention due to the elevation in its mRNA level after treatment with Interleukin-1 (7). However, in a mouse model of osteoarthritis, ADAMTS4 knock-out mice did not exhibit any significant protective effect (8). ADAMTS4 consists of a signal peptide (aa 1 to 51), a pro domain (aa 52 to 212) and a mature chain (aa 213 to 837) containing the following domains: catalytic (aa 218 to 425), disintegrin (aa 437 to 519), TSP type-1 (aa 520 to 575), Cys-rich (aa 577 to 685) and spacer (aa 686 to 837). The amino acid sequence of rhADAMTS4 is 100%, 97% and 94% identical to that of chimpanzee, dog, and mouse/rat/bovine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.