Activin RIA/ALK-2/Activin Receptor Type 1 Antibody

Product Discontinued

Activin RIA/ALK-2/Activin Receptor Type 1 Antibody Summary

• Immunogen: Recombinant fragment (Human).
• Specificity: The exhibits approximately 10 % cross-reactivity with recombinant human activin receptor IIA and recombinant human activin receptor IIB.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACVR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1-0.2 ug/ml
• Application Notes: WB: Detects human activin receptor IA at approximately 5 ng/lane under reducing and non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Reactivity Notes

Cross-reacts with Human.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody

• activin A receptor, type I
• Activin RIA
• ActivinRIA
• ACTRI
• ACVR1
• ACVR1A
• ACVRLK2
• ALK2
• ALK-2
• FOP
• SKR1
• TSRI

Background

Activin, a disulfide-linked dimeric protein is secreted by Sertoli cells in the testis and grandulosa cells in the ovary. In early studies, this peptide was thought to be an inhibin and not recognized as a unique compound. Activins and inhibins are members of the TGF-beta superfamily due to amino acid homology with respect to the conservation of of the cysteine residues common to all TGF-beta forms. Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits have been cloned (mammalian betaA, betaB, betaC, betaE, and Xenopus betaD). The activin/inhibin nomenclature reflects the subunit composition of the proteins: activin A (betaA-betaA), activin B (betaB-betaB), activin AB (betaB-betaA), inhibin A (alpha-betaA), and inhibin B (alpha-betaB). Activins have a wide range of biological activities including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis, and reproductive physiology. Activin A is involved in growth and differentiation of several tissues from different species. This protein also plays a key role in the production and regulation of hormones such as FSH, LH, GnRH, and ACTH. Activin influences erythropoiesis and the potentiation of erythroid colony formation, oxytocin secretion, paracrine, and autocrine regulation. Similar to other TGF-beta family members, activins exert their biological activities through the effects of the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II receptors. Activin type I and type II receptors are distinguished by the level of sequence homology of their kinase domains and other structural and functional features. To date, seven type I and five type II activin receptors have been cloned from mammals, including activin receptor IA, activin receptor IIA, activin receptor IB, and activin receptor IIB. In addition, two splice variants of activin receptor IIA and five splice variants of activin receptor IIB have been reported. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Activin receptor IA is highly conserved and will also bind to form signaling complexes with the BMP-2/7-bound BMPR-II. Human, mouse, and bovine type IA activin receptors share greater than 98 % homology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACVR1
• Entrez:
  • 90(Human)