Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 71412
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Alexa Fluor® 405] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK‑2
  Asp23-Val124
  Accession # Q04771
• Specificity: Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Activin RIA/ALK-2/Activin Receptor Type 1 Antibody (71412) [Alexa Fluor® 405]

• activin A receptor, type I
• Activin RIA
• ActivinRIA
• ACTRI
• ACVR1
• ACVR1A
• ACVRLK2
• ALK2
• ALK-2
• FOP
• SKR1
• TSRI

Background

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.