active/cleaved Caspase-3 Assay Kit (Fluorometric)

Product Discontinued

active/cleaved Caspase-3 Assay Kit (Fluorometric) Summary

• Description: Activation of caspases plays a central role in apoptosis. The Active Caspase-3 Staining Kit provides a convenient means for detecting activated caspase-3 in living cells. The assay utilizes the caspase-3 inhibitor DEVD-FMK conjugated to sulfo-rhodamine (Red-DEVD-FMK) as the fluorescent in situ marker. Red-DEVD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells.
  
  Detection method: Flow cytometry (FL-2 channel), fluorescence microscopy, fluorescence plate reader (Ex/Em = 540/570 nm).Applications: Detection of activated caspase-3 in living cells.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65617)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP3

Applications/Dilutions

• Dilutions:
  • Flow Cytometry

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for active/cleaved Caspase-3 Assay Kit (Fluorometric)

• Apopain
• apoptosis-related cysteine protease
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• caspase-3
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• Yama

Background

Caspase 3 (CASP3) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis.

There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Caspase 3 is considered an effector, as it is activated in the apoptotic cell both by extrinsic (death ligand) and intrinsic (mitochondrial) pathways. Specifically, procaspase is activated when the procaspase is cleaved at a particular residue. Activated Caspase 3 then cleaves numerous substrates to effect the morphological changes associated with apoptosis.

Caspase 3 antibodies are important tools for apoptosis studies and research on the caspase family mediated apoptosis signaling pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP3