Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [HRP]

• Reactivity: Hu, Rt, Ca, Fe, Rb
• Applications: Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
• Clone: HHF35 + MSA/953
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: HRP

Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [HRP] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Recombinant human Actin (Muscle Specific) fragment
• Localization: Cytoplasmic
• Marker: Muscle Cell Marker
• Specificity: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACTA1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NBP2-47663. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for Actin (Muscle Specific) Antibody (HHF35 + MSA/953) [HRP]

• ACTA
• ACTC
• actin, alpha 2, smooth muscle, aorta
• actin, alpha cardiac muscle 1
• actin, alpha skeletal muscle
• actin, aortic smooth muscle
• ACTSA
• alpha-cardiac actin
• ASD5
• ASMA
• Cell growth-inhibiting gene 46 protein
• CFTD
• CFTD1
• CFTDM
• CMD1R
• CMH11
• MPFD
• NEM1
• NEM2
• NEM3
• nemaline myopathy type 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ACTA1
• Uniprot:
  • P68032()
  • P62736(Human)
  • P68133(Human)