Recombinant Human ACOT8 His Protein

SDS-Page: ACOT8 Protein [NBP2-22676]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human ACOT8 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-319 of Human ACOT8
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSSPQAP EDGQGCGDRG DPPGDLRSVL VTTVLNLEPL DEDLFRGRHY WVPAKRLFGG QIVGQALVAA AKSVSEDVHV HSLHCYFVRA GDPKLPVLYQ VERTRTGSSF SVRSVKAVQH GKPIFICQAS FQQAQPSPMQ HQFSMPTVPP PEELLDCETL IDQYLRDPNL QKRYPLALNR IAAQEVPIEI KPVNPSPLSQ LQRMEPKQMF WVRARGYIGE GDMKMHCCVA AYISDYAFLG TALLPHQWQH KVHFMVSLDH SMWFHAPFRA DHWMLYECES PWAGGSRGLV HGRLWRQDGV LAVTCAQEGV IRVKPQVSES KL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: ACOT8
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 38.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 2 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human ACOT8 His Protein

• ACTEIII
• acyl-CoA thioesterase 8HNAACTE
• Choloyl-coenzyme A thioesterase
• EC 3.1.2.27
• hACTEIII
• HIV-Nef-associated acyl-CoA thioesterase
• hTEcholoyl-CoA hydrolase
• long-chain fatty-acyl-CoA hydrolase
• palmitoyl-CoA hydrolase
• peroxisomal acyl-CoA thioesterase 1
• peroxisomal acyl-CoA thioesterase
• Peroxisomal acyl-coenzyme A thioester hydrolase 1
• Peroxisomal long-chain acyl-CoA thioesterase 1
• PTE-1
• PTE1HIV-Nef associated acyl-CoA thioesterase
• PTE2
• PTE-2hACTE-IIIacyl-coenzyme A thioesterase 8
• Thioesterase II
• thioesterase III

Background

Acyl-coenzyme A thioesterase 8, also known as ACOT8, are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. This protein may mediate Nef-induced down-regulation of CD4. It competes with BAAT (Bile acid CoA: amino acid N-acyltransferase) for bile acid-CoA substrate (such as chenodeoxycholoyl-CoA). ACOT8 shows a preference for medium-length fatty acyl-CoAs. Recombinant human ACOT8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: ACOT8