ACMSD Antibody - BSA Free

Western Blot: ACMSD Antibody [NBP1-33499] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ACMSD antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Paraffin: ACMSD Antibody [NBP1-33499] - SAS xenograft, using ACMSD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Western Blot: ACMSD Antibody [NBP1-33499] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.

Product Discontinued

ACMSD Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ACMSD Antibody - BSA Free (NBP1-33499) is a polyclonal antibody validated for use in IHC and WB. Anti-ACMSD Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ACMSD. The exact sequence is proprietary.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ACMSD
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ACMSD Antibody - BSA Free

• aminocarboxymuconate semialdehyde decarboxylase
• EC 4.1.1.45,2-amino-3-carboxymuconate-6-semialdehyde decarboxylase
• Picolinate carboxylase

Background

The neuronal excitotoxin quinolinate is an intermediate in the de novo synthesis pathway of NAD from tryptophan, and has been implicated in the pathogenesis of several neurodegenerative disorders. Quinolinate is derived from alpha-amino-beta-carboxy-muconate-epsilon-semialdehyde (ACMS). ACMSD (ACMS decarboxylase; EC 4.1.1.45) can divert ACMS to a benign catabolite and thus prevent the accumulation of quinolinate from ACMS.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for ACMSD Antibody (NBP1-33499)(2)

Publications using NBP1-33499

• Zeidler J, Chini C, Kanamori K et al. Endogenous metabolism in endothelial and immune cells generates most of the tissue vitamin B3 (nicotinamide) iScience 2022-10-01 [PMID: 36388973] (WB, Human)
• Palzer L, Bader JJ, Angel F et al. Alpha-Amino-Beta-Carboxy-Muconate-Semialdehyde Decarboxylase Controls Dietary Niacin Requirements for NAD+ Synthesis. Cell reports. 1905-07-10 [PMID: 30380424] (WB, Mouse)

Bioinformatics

• Gene Symbol: ACMSD