ACAT1 Antibody

Western Blot: ACAT1 Antibody [NBP1-51994] - Analysis of Mouse (A) and Rat (B) kidney lysates, and Mouse (C) and Rat (D) Liver lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: ACAT1 Antibody [NBP1-51994] - (0.01ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACAT1 Antibody Summary

• Description: Novus Biologicals Goat ACAT1 Antibody (NBP1-51994) is a polyclonal antibody validated for use in WB and ELISA. Anti-ACAT1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KRVDFSKVPKLKT corresponding to internal region according to NP_000010.1.
• Epitope: C-KRVDFSKVPKLKT
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: IF: An anonymous customer has reported particulate cytoplasm staining in HeLa WB: Approx. 45 kDa band observed in human liver lysates and in rodent kidney and liver lysates (calculated MW of 45.2 kDa band according to NP_000010.1).
• Theoretical MW: 45.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACAT1 Antibody

• ACAT
• ACAT1
• acetoacetyl Coenzyme A thiolase
• Acetoacetyl-CoA thiolase
• acetyl-CoA acetyltransferase 1
• acetyl-CoA acetyltransferase, mitochondrial
• acetyl-Coenzyme A acetyltransferase 1
• EC 2.3.1
• EC 2.3.1.9
• MAT
• mitochondrial acetoacetyl-CoA thiolase
• T2
• THIL

Background

ACAT-1 is a ~50 kDa protein that catalyzes the formation of intracellular cholesterol esters in various tissues, such as adult human liver, adrenal glands, macrophages, and kidneys.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACAT1 Antibody (NBP1-51994)(1)

Publication using NBP1-51994

• Hongo S, Watanabe T, Arita S et al. Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. Am J Physiol Cell Physiol 2009-08-01 [PMID: 19625677]

Bioinformatics

• Gene Symbol: ACAT1
• Entrez:
  • 38(Human)
• Uniprot:
  • NP_000010.1(Human)