Abraxas Antibody (ABRA1-01)

Western Blot: Abraxas Antibody (ABRA1-01) [NBP1-69762] - Detection of ABRA1-EGFP fusion protein in lysate of transfected HEK293 cells. Lane A: Immunostaining with anti-ABRA1 (clone ABRA1-01). Lane B: Immunostaining with anti-EGFP (polyclonal). Lane C: Immunostaining with anti-alpha tubulin (clone TU-01).

Immunohistochemistry-Paraffin: Abraxas Antibody (ABRA1-01) [NBP1-69762] - Analysis of anti-FAM175A / CCDC98 antibody with human testis at concentration 10 ug/ml.

Product Discontinued

Abraxas Antibody (ABRA1-01) Summary

• Immunogen: Recombinant protein corresponding to amino acids 1-313 of Abraxas with N-terminal His6 tag.
• Specificity: Abraxas Antibody recognizes N-terminal part of Abraxas (CCDC98), an adaptor protein involved in DNA repair, which migrates as a 45 kD band on PAAGE under reducing conditions.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABRAXAS1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1 - 2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Abraxas Antibody (ABRA1-01)

• BRCA1-A complex subunit Abraxas
• ABRA1
• CCDC98
• family with sequence similarity 175, member A

Background

Abraxas was identified in a phosphopeptide affinity proteomic and mass spec analysis as a protein that bound directly to the BRCA1 BRCT repeat. Along with RAP80, Abraxas was required to form a complex with BRCA1 for the DNA damage response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABRAXAS1
• Entrez:
  • 84142(Human)
• Uniprot:
  • Q6UWZ7(Human)