ABI1 Overexpression Lysate (Native)

Images

 
Western Blot: ABI1 Overexpression Lysate (Adult Normal) [NBL1-07203] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ABI1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ABI1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC217707 and based on accession number NM_005470. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens abl-interactor 1 (ABI1), transcript variant 1, mRNA.
Gene
ABI1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
54.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for ABI1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ABI1 Overexpression Lysate (Native)

  • Abelson interactor 1
  • ABI-1
  • abl interactor 1
  • Abl-binding protein 4
  • ablBP4
  • abl-interactor 1
  • Abl-interactor protein 1 long
  • E3B1
  • eps8 binding protein
  • Eps8 SH3 domain-binding protein
  • Eps8-binding protein
  • interactor protein AblBP4
  • nap1 binding protein
  • Nap1-binding protein
  • Nap1BP
  • spectrin SH3 domain binding protein 1
  • Spectrin SH3 domain-binding protein 1
  • SSH3BP
  • SSH3BP1

Background

The protein encoded by this gene has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, the encoded protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling. Several transcript variants encoding multiple isoforms have been found for this gene. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ABI1 Lysate (NBL1-07203) (0)

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Bioinformatics

Gene Symbol ABI1