ABHD14B Antibody (011) - Azide and BSA Free

Western Blot: ABHD14B Antibody (011) [NBP2-90260] - Anti-ABHD14B rabbit monoclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Predicted band size:22 kDa. Observed band size:23 kDa.

Immunoprecipitation: ABHD14B Antibody (011) [NBP2-90260] - ABHD14B was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg Hela Whole Cell Lysate. 0.5 uL anti-ABHD14B rabbit monoclonal antibody and 15 ul of 50% Protein G agarose. Primary antibody: Anti-ABHD14B rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 25 kDa. Observed band size: 25 kDa

• Reactivity: Hu
• Applications: WB, IP
• Clone: 011
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ABHD14B Antibody (011) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was obtained from a rabbit immunized with purified, recombinant Human ABHD14B (Uniprot#: Q96IU4-1; Met1-Gln210).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ABHD14B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 0.2-1 uL/mg of lysate
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for ABHD14B Antibody (011) - Azide and BSA Free

• abhydrolase domain containing 14B
• abhydrolase domain-containing protein 14B
• CCG1-interacting factor B
• CIBcell cycle gene 1-interacting factor B
• EC 3.-
• EC 3.1.11.6
• EC 3.1.21.4
• MGC15429

Background

ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro). May activate transcription

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ABHD14B