ABCG2/CD338 Antibody (BXP-21)

Immunohistochemistry: ABCG2/CD338 Antibody (BXP-21) [NB100-2177] - Tissue was fixed with formaldehyde, blocked with 3% H2O2 for 5 minutes at 20 C and permeabilized with PBS-Tween; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/200 in PBS-Tween) for 1 hour at 20 C. A biotinylated goat anti-mouse polyclonal IgG was used as the secondary antibody.

Chromatin Immunoprecipitation: ABCG2/CD338 Antibody (BXP-21) [NB100-2177] - Staining in MCF7 human breast cancer cells. Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were then blocked using 10% serum for 2 hours at 25 C.

Product Discontinued

ABCG2/CD338 Antibody (BXP-21) Summary

• Immunogen: Fusion protein composed of E. Coli maltose binding protein and BCRP peptide [126 amino acids, 271-396 AA of BCRP.
• Localization: Plasma membrane; integral membrane protein
• Marker: Stem Cell Marker
• Specificity: This recognizes an internal epitope of human BCRP (70 kDa), a transmembrane half-transporter. The does not cross-react with the human MDR1, MRP1, and MRP2 gene products.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCG2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence 1:20-1:50
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:20-1:40
  • Immunohistochemistry-Paraffin 1:20-1:40
  • Western Blot 1:100-1:2000
• Application Notes: ICC: Using immunocytochemistry on acetone-fixed, frozen cytospin preparations. IHC: Using immunohistochemistry on acetone-fixed, frozen sections using rabbit anti-mouse IgG and a APAAP complex. The antibody can be used on formalin-fixed, paraffinembedded human tissues and tumors after pretreatment with 10 mM citric acid, pH 6.0, for 20 minutes. WB: Use at assay dependant dilution.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29978425).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Serum - free Medium with 0.7% BSA
• Preservative: 0.1% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Tissue culture supernatant

Alternate Names for ABCG2/CD338 Antibody (BXP-21)

• ABC transporter
• ABC15
• ABCG2
• ABCP
• ABCPMGC102821
• ATP-binding cassette sub-family G member 2
• ATP-binding cassette transporter G2
• ATP-binding cassette, sub-family G (WHITE), member 2
• BCRP
• BCRP1
• BCRPMRX
• BMDP
• Breast cancer resistance protein
• CD338 antigen
• CD338
• CDw338
• EST157481
• mitoxantrone resistance protein
• Mitoxantrone resistance-associated protein
• MXR
• MXRMXR1
• placenta specific MDR protein
• Placenta-specific ATP-binding cassette transporter

Background

Tumor cells may display a multidrug resistant phenotype by overexpression of ATP-binding cassette transporters such as multidrug resistance (MDRI) p-glycoprotein, multidrug resistance protein 1 (MRP1), and breast cancer resistance protein (BCRP). Tumor cells can be intrinsically resistant to drugs or they can acquire resistance to structurally and functionally unrelated drugs on drug exposure. This phenomenon is known as multidrug resistance (MDR). In human tumor cells, several transporter proteins can be involved in MDR. These proteins, MDR1 P-gp (ABCB1), MRP1 (ABCC1), MRP2 (ABCC2), MRP3 (ABCC3), and BCRP (ABCG2), belong to the ABC transporter family. They act as efflux pumps, which result in decreased intracellular concentrations of cytotoxic drugs. BCRP is a recently discovered half-transporter that probably acts as a homo- or heterodimer in transporting cytotoxic agents. The transporter molecule is capable of transporting several anticancer drugs but has thus far been found mainly in MX-resistant cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG2/CD338 Antibody (NB100-2177)(8)

Publications using NB100-2177

• Doganlar O, Doganlar ZB, Erdogan S, Delen E Antineoplastic multi-drug chemotherapy to sensitize tumors triggers multi-drug resistance and inhibits efficiency of maintenance treatment inglioblastoma cells EXCLI journal 2023-01-04 [PMID: 36660193] (WB, Human)
• Hussein N, Ashby CR, Amawi H et al. Cariprazine, A Dopamine D2/D3 Receptor Partial Agonist, Modulates ABCG2-Mediated Multidrug Resistance in Cancer. Cancers (Basel) 2018-09-04 [PMID: 30181510] (WB, Human)
• Hammad AM, Alasmari F, Sari Y et al. Alcohol and Cocaine Exposure Modulates ABCB1 and ABCG2 Transporters in Male Alcohol-Preferring Rats. Mol. Neurobiol. 2018-07-06 [PMID: 29978425] (WB, Rat)
• Hussein N, Amawi H, Karthikeyan C et al. The dopamine D3 receptor antagonists PG01037, NGB2904, SB277011A, and U99194 reverse ABCG2 transporter-mediated drug resistance in cancer cell lines. Cancer Lett. 2017-03-18 [PMID: 28323029] (ICC/IF)
• Liang SC, Yang CY, Tseng JY et al. ABCG2 Localizes to the Nucleus and Modulates CDH1 Expression in Lung Cancer Cells. Neoplasia. 2015-03-01 [PMID: 25810011] (IHC-P, Human)
• Venkatraman G, Benesch Mg, Tang X et al. Lysophosphatidate signaling stabilizes nrf2 and increases the expression of genes involved in drug resistance and oxidative stress responses: implications for cancer treatment. FASEB J. 2014-11-14 [PMID: 25398768] (WB, Human)
• Svirnovski AI, Shman TV, Serhiyenka TF et al. ABCB1 and ABCG2 proteins, their functional activity and gene expression in concert with drug sensitivity of leukemia cells. Hematology 2009-08-01 [PMID: 19635183]
• Ejendal, K et al. The nature of amino acid 482 of human ABCG2 affects substrate transport ATP hydrolysis but not substrate binding. Protein Sci, 15: 1597-1607. 2006-07-01 [PMID: 16815914]

Review for ABCG2/CD338 Antibody (NB100-2177) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ewa Kaniewska IHC Human 05/13/2014

Summary

• Application: Immunohistochemistry
• Sample Tested: Human cells
• Species: Human
• Lot: SLBF7613

Bioinformatics

• Gene Symbol: ABCG2
• Entrez:
  • 9429(Human)
• Uniprot:
  • Q9UNQ0(Human)