ABCG2/CD338 Antibody (5D3) [Phycoerythrin]

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ABCG2 PE-conjugated Monoclonal Antibody (Catalog # FAB995P, filled histogram) or isotype control antibody (IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Freshly isolated aortic pVICs express distinct cell surface markers.Freshly isolated aortic pVICs were stained with antibodies for CD31, OB–CDH, ABCG2, NG2, SSEA-4, and the corresponding control antibodies. Staining was quantified by flow cytometry. In the figure, the y-axis is fluorescence intensity and the x-axis is forward scattering. Percentage in the rectangular gates represents the fraction of positively stained cells. After subtracting the background, about 7.70% of these aortic pVICs stained positive for CD31, 4.71% stained positive for OB–CDH, 5.60% stained positive for ABCG2, 5.56% stained positive for NG2, and 6.59% stained positive for SSEA-4. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0069667), licensed under a CC-BY license. Not internally tested by R&D Systems.

RPMI 8226 cells were stained with Mouse Anti-Human ABCG2 PE‑conjugated Monoclonal Antibody (Catalog # FAB995P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 5D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

ABCG2/CD338 Antibody (5D3) [Phycoerythrin] Summary

• Immunogen: 3T3 cells transduced with human ABCG2
• Specificity: Detects human ABCG2 in flow cytometry and immunocytochemistry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ABCG2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ABCG2/CD338 Antibody (5D3) [Phycoerythrin]

• ABC transporter
• ABC15
• ABCG2
• ABCP
• ABCPMGC102821
• ATP-binding cassette sub-family G member 2
• ATP-binding cassette transporter G2
• ATP-binding cassette, sub-family G (WHITE), member 2
• BCRP
• BCRP1
• BCRPMRX
• BMDP
• Breast cancer resistance protein
• CD338 antigen
• CD338
• CDw338
• EST157481
• mitoxantrone resistance protein
• Mitoxantrone resistance-associated protein
• MXR
• MXRMXR1
• placenta specific MDR protein
• Placenta-specific ATP-binding cassette transporter

Background

Hematopoietic stem cells are known to express a membrane transporter molecule, known as P-glycoprotein (Pgp), that is encoded by the multidrug resistance gene 1 (MDR1) (1, 2). Expression of Pgp appears to confer a proliferative advantage to stem cells through its anti-apoptotic effects (3, 4). An additional transporter molecule known as ABCG2 (ATP-binding cassette gene 2) or Bcrp1 (Breast cancer resistance protein 1), first identified in a breast cancer cell line (5), is expressed on stem cells (6). ABCG2 belongs to a family of molecules that span the cell membrane six times and can exist as either homo or hetero dimers linked by a short intracellular flexible linker region that plays an important role in the efflux of a wide range of substrates (7, 8). Although these transporter molecules have initially been thought to play a role in drug resistance, they have been found to have utility in better characterizing primitive stem cells. For example, the “side-population” of hematopoietic stem cells, characterized by their inability to retain high levels of the intracellular staining dyes Hoechst 33342 and Rhodamine 123, has been found to express high levels of ABCG2. Of interest is the observation that ABCG2 function has been linked to the efflux of the Hoechst dye (6). Furthermore, there is now evidence that this monoclonal can be used as a cell surface marker to identify hematopoietic stem cells within the bone marrow fraction of lineage negative cells (6). The expression of ABCG2 appears greatest on CD34^- cells and is downregulated with the acquisition of CD34 on the cell surface (6).

1. Chaudhary, P.M. and I.B. Roninson (1991) Cell 66:85.
2. Sorrentino, B.P. et al. (1995) Blood 86:491.
3. Pallis, M. and N. Russell (2000) Blood 95:2897.
4. Johnstone, R.W. et al. (1999) Blood 93:1075.
5. Doyle, L.A. et al. (1998) Proc. Natl. Acad. Sci. USA 95:15665.
6. Zhou, S. et al. (2001) Nat. Medicine 7:1028.
7. Hrycyna, C.A. et al. (1998) Biochem. 37:13660.
8. Bunting, K.D. (2002) Stem Cells 20:11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ABCG2/CD338 Antibody (FAB995P)(11)

Publications using FAB995P

• Leow, BCS;Kok, CH;Yeung, DT;Hughes, TP;White, DL;Eadie, LN; The acquisition order of leukemic drug resistance mutations is directed by the selective fitness associated with each resistance mechanism Scientific reports 2023-08-11 [PMID: 37567965] (Flow Cytometry, Human)
• MM Kutyna, S Loone, VA Saunders, DL White, CH Kok, DK Hiwase Solute Carrier Family 29A1 Mediates In Vitro Resistance to Azacitidine in Acute Myeloid Leukemia Cell Lines International Journal of Molecular Sciences, 2023-02-10;24(4):. 2023-02-10 [PMID: 36834962] (Flow Cytometry, Human)
• S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium Stem Cell Res Ther, 2019-11-04;10(1):316. 2019-11-04 [PMID: 31685023] (Flow Cytometry, Human)
• M Ding, H Zhang, L Liu, R Liang Effect of NOS1 regulating ABCG2 expression on proliferation and apoptosis of cervical cancer cells Oncol Lett, 2018-11-30;17(2):1531-1536. 11/30/2018 [PMID: 30675209]
• JT Szilagyi, AM Vetrano, JD Laskin, LM Aleksunes Localization of the placental BCRP/ABCG2 transporter to lipid rafts: Role for cholesterol in mediating efflux activity Placenta, 2017-04-12;55(0):29-36. 2017-04-12 [PMID: 28623970] (Flow Cytometry, Human)
• R Bayat Mokh, N Baluch, M Ka Hon Tsu, S Kumar, T S Homayoun, K Aitken, B Das, S Baruchel, H Yeger Acetazolamide potentiates the anti-tumor potential of HDACi, MS-275, in neuroblastoma BMC Cancer, 2017-02-24;17(1):156. 2017-02-24 [PMID: 28235409] (Flow Cytometry, Human)
• Boyle M, Chun C, Strojny C, Narayanan R, Bartholomew A, Sundivakkam P, Alapati S Chronic inflammation and angiogenic signaling axis impairs differentiation of dental-pulp stem cells. PLoS ONE, 2014-11-26;9(11):e113419. 2014-11-26 [PMID: 25427002] (Flow Cytometry, Human)
• Huan Wang, Balaji Sridhar, Leslie A. Leinwand, Kristi S. Anseth Characterization of Cell Subpopulations Expressing Progenitor Cell Markers in Porcine Cardiac Valves PLoS ONE 2013-07-23 [PMID: 23936071]
• Kondo S, Wakisaka N, Muramatsu M Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines. J. Virol., 2011-08-17;85(21):11255-64. 2011-08-17 [PMID: 21849440] (Flow Cytometry, Human)
• The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells. Biochem. Biophys. Res. Commun., 2010-08-20;400(0):334-9. 2010-08-20 [PMID: 20728430] (Flow Cytometry, Human)
• Nakanishi T, Chumsri S, Khakpour N, Brodie AH, Leyland-Jones B, Hamburger AW, Ross DD, Burger AM Side-population cells in luminal-type breast cancer have tumour-initiating cell properties, and are regulated by HER2 expression and signalling. Br. J. Cancer, 2010-02-09;102(5):815-26. 2010-02-09 [PMID: 20145614] (Flow Cytometry, Human)

Review for ABCG2/CD338 Antibody (FAB995P) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Other.

reviewed by: Verified Customer Flow Other 01/27/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 23936071
• Species: Other

Bioinformatics

• Gene Symbol: ABCG2